1qoq: Difference between revisions
New page: left|200px<br /> <applet load="1qoq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qoq, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1QOQ is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with IGP and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QOQ OCA]]. | 1QOQ is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with IGP and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Lyase Lyase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Site: IGP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QOQ OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 23: | Line 23: | ||
[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:36:27 2007'' | ||
Revision as of 09:31, 30 October 2007
|
CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH INDOLE GLYCEROL PHOSPHATE
OverviewOverview
We used freeze trapping to stabilize the Michaelis complex of wild-type, tryptophan synthase and the alpha-subunit substrate indole-3-glycerol, phosphate (IGP) and determined its structure to 1. 8 A resolution. In, addition, we determined the 1.4 A resolution structure of the complex with, indole-3-propanole phosphate (IPP), a noncleavable IGP analogue. The, interaction of the 3'-hydroxyl of IGP with the catalytic alphaGlu49 leads, to a twisting of the propane chain and to a repositioning of the indole, ring compared to IPP. Concomitantly, the catalytic alphaAsp60 rotates, resulting in a translocation of the COMM domain [betaGly102-betaGly189, for definition see Schneider et al. (1998) Biochemistry 37, 5394-5406] in, a direction opposite to the one in the IPP complex. This results in ... [(full description)]
About this StructureAbout this Structure
1QOQ is a [Protein complex] structure of sequences from [Salmonella typhimurium] with IGP and PLP as [ligands]. Active as [Lyase], with EC number [4.2.1.20]. Structure known Active Site: IGP. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of wild-type tryptophan synthase complexed with the natural substrate indole-3-glycerol phosphate., Weyand M, Schlichting I, Biochemistry. 1999 Dec 14;38(50):16469-80. PMID:10600108
Page seeded by OCA on Tue Oct 30 08:36:27 2007