2cfp: Difference between revisions
New page: left|200px<br /> <applet load="2cfp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cfp, resolution 3.30Å" /> '''SUGAR FREE LACTOSE ... |
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==About this Structure== | ==About this Structure== | ||
2CFP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with HG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA]]. | 2CFP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with HG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: transport mechanism]] | [[Category: transport mechanism]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:36:23 2007'' | ||
Revision as of 09:31, 30 October 2007
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SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH
OverviewOverview
Cation-coupled active transport is an essential cellular process found, ubiquitously in all living organisms. Here, we present two novel, ligand-free X-ray structures of the lactose permease (LacY) of Escherichia, coli determined at acidic and neutral pH, and propose a model for the, mechanism of coupling between lactose and H+ translocation. No, sugar-binding site is observed in the absence of ligand, and deprotonation, of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the, initial step in H+ transduction.
About this StructureAbout this Structure
2CFP is a [Single protein] structure of sequence from [Escherichia coli] with HG as [ligand]. Structure known Active Site: AC2. Full crystallographic information is available from [OCA].
ReferenceReference
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509
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