5lip: Difference between revisions
New page: left|200px<br /> <applet load="5lip" size="450" color="white" frame="true" align="right" spinBox="true" caption="5lip, resolution 2.9Å" /> '''PSEUDOMONAS LIPASE C... |
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==About this Structure== | ==About this Structure== | ||
5LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and OCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5LIP OCA]]. | 5LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and OCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Structure known Active Sites: ACT and OXY. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5LIP OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: triglyceride analogue]] | [[Category: triglyceride analogue]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:34:56 2007'' | ||
Revision as of 09:30, 30 October 2007
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PSEUDOMONAS LIPASE COMPLEXED WITH RC-(RP, SP)-1,2-DIOCTYLCARBAMOYLGLYCERO-3-O-OCTYLPHOSPHONATE
OverviewOverview
To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and, Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates, of different alkyl chain lengths. P. cepacia lipase was most rapidly, inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl, octylphosphonate (Rc-trioctyl) with an inactivation half-time of 75 min, while that for the, Sc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octyl-phosphonate, (Sc-trioctyl) compound was 530 min. X-ray structures were obtained of P., cepacia lipase after reaction with Rc-trioctyl to 0.29-nm resolution at pH, 4 and covalently modified with, Rc-(Rp,Sp)-1,2-dibutylcarbamoylglycero-3-O-p-nitrophenyl butyl-phosphonate, (Rc-tributyl) to 0.175-nm resolution ... [(full description)]
About this StructureAbout this Structure
5LIP is a [Single protein] structure of sequence from [Burkholderia cepacia] with CA and OCP as [ligands]. Active as [Hydrolase], with EC number [3.1.1.3]. Structure known Active Sites: ACT and OXY. Full crystallographic information is available from [OCA].
ReferenceReference
Structural basis of the chiral selectivity of Pseudomonas cepacia lipase., Lang DA, Mannesse ML, de Haas GH, Verheij HM, Dijkstra BW, Eur J Biochem. 1998 Jun 1;254(2):333-40. PMID:9660188
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