The Bacterial Flagellar Hook: Difference between revisions
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==Structure of the Hook Monomer, FlgE== | ==Structure of the Hook Monomer, FlgE== | ||
<applet load='1wlg' size='300' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/ | <applet load='1wlg' size='300' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/3'/> | ||
The hook is composed of about 120 copies of the protein chain FlgE (the product of the [http://ecoliwiki.net/colipedia/index.php/flgE:Quickview gene flgE]). In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. FlgE has 402 amino acids. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | The hook is composed of about 120 copies of the protein chain FlgE (the product of the [http://ecoliwiki.net/colipedia/index.php/flgE:Quickview gene flgE]). In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. FlgE has 402 amino acids. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | ||
FlgE consists of three domains, D0, D1, and D2. The (<scene name='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/ | FlgE consists of three domains, D0, D1, and D2. The (<scene name='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/3'>initial scene</scene>) at right shows D1 and D2 (D0 having been deleted in order to achieve crystallization, as mentioned above). | ||
{{Template:ColorKey_Amino2CarboxyRainbow}} | {{Template:ColorKey_Amino2CarboxyRainbow}} | ||
Note that D2 consists of a continuous segment of the protein chain, while D1 is made up of discontinuous segments. D0 (not shown, see above) is attached to D1, and D0 forms the inner channel of the hook. D2 forms the outer surface of the hook. | Note that D2 consists of a continuous segment of the protein chain, while D1 is made up of discontinuous segments. D0 (not shown, see above) is attached to D1, and D0 forms the inner channel of the hook. D2 forms the outer surface of the hook. | ||