The Bacterial Flagellar Hook: Difference between revisions
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Eric Martz (talk | contribs) →Structure of the Hook Monomer, FlgE: polishing |
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The hook is composed of about 120 copies of the protein chain FlgE (the product of the [http://ecoliwiki.net/colipedia/index.php/flgE:Quickview gene flgE]). In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. FlgE has 402 amino acids. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length protein). Removal of the ends of the full-length chain was required in order to coax the protein to crystallize, instead of forming filaments. | The hook is composed of about 120 copies of the protein chain FlgE (the product of the [http://ecoliwiki.net/colipedia/index.php/flgE:Quickview gene flgE]). In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. FlgE has 402 amino acids. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length protein). Removal of the ends of the full-length chain was required in order to coax the protein to crystallize, instead of forming filaments. | ||
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