1e4y: Difference between revisions
New page: left|200px<br /> <applet load="1e4y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e4y, resolution 1.85Å" /> '''MUTANT P9L OF ADENY... |
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==About this Structure== | ==About this Structure== | ||
1E4Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with AP5 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E4Y OCA]]. | 1E4Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with AP5 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3]]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E4Y OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase(phosphotransferase)]] | [[Category: transferase(phosphotransferase)]] | ||
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Revision as of 09:17, 30 October 2007
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MUTANT P9L OF ADENYLATE KINASE FROM E. COLI, MODIFIED IN THE GLY-LOOP
OverviewOverview
Two mutants of adenylate kinase from Escherichia coli have been, crystallized and analyzed by X-ray diffraction at resolutions of 3.4 and, 2.4 A, respectively. These mutants are Pro-9-->Leu and Gly-10-->Val. They, were selected for their positions in the highly conserved Gly-loop forming, a giant anion hole for the beta-phosphate of ATP (GTP) in adenylate, kinases, H-ras-p21, and other nucleotide-binding proteins. Mutants at, these positions of H-ras-p21 cause cancer. In adenylate kinase these, mutations cause smallish changes at the active site. Relating the, structural changes to the known changes in catalysis indicates that these, mutants hinder the induced-fit movements. As a side result we find that, mutant Pro-9-->Leu and wild-type form one very similar crystal packing, ... [(full description)]
About this StructureAbout this Structure
1E4Y is a [Single protein] structure of sequence from [Escherichia coli] with AP5 as [ligand]. Active as [Transferase], with EC number [2.7.4.3]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structures of two mutants of adenylate kinase from Escherichia coli that modify the Gly-loop., Muller CW, Schulz GE, Proteins. 1993 Jan;15(1):42-9. PMID:8451239
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