User:Yana Fedotova/Sandbox 1: Difference between revisions
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Molecular weight: 20483.5 Da | *Molecular weight: 20483.5 Da | ||
Number of Amino Acids: 184 | *Number of Amino Acids: 184 | ||
Theoretical pI: 6.85 | *Theoretical pI: 6.85 | ||
The estimated half-life is: | *The estimated half-life is: | ||
30 hours (mammalian reticulocytes, in vitro). | |||
>20 hours (yeast, in vivo). | |||
>10 hours (Escherichia coli, in vivo). | |||
Total number of negatively charged residues (Asp + Glu): 28 | *Total number of negatively charged residues (Asp + Glu): 28 | ||
Total number of positively charged residues (Arg + Lys): 28 | *Total number of positively charged residues (Arg + Lys): 28 | ||
Atomic | '' | ||
Carbon C 894 | == Atomic Composition == | ||
Hydrogen H 1468 | '' | ||
Nitrogen N 268 | |||
Oxygen O 268 | Carbon C 894 | ||
Sulfur S 7 | Hydrogen H 1468 | ||
Nitrogen N 268 | |||
Oxygen O 268 | |||
Sulfur S 7 | |||
Total number of atoms: 2905 | *Total number of atoms: 2905 | ||
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. | *Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. | ||
Ext. coefficient 11585 | Ext. coefficient 11585 | ||
Abs 0.1% (=1 g/l) 0.566, assuming ALL Cys residues appear as half cystines | *Abs 0.1% (=1 g/l) 0.566, assuming ALL Cys residues appear as half cystines | ||
Ext. coefficient 11460 | Ext. coefficient 11460 | ||
Abs 0.1% (=1 g/l) 0.559, assuming NO Cys residues appear as half cystines | *Abs 0.1% (=1 g/l) 0.559, assuming NO Cys residues appear as half cystines | ||
Instability index: 55.68 | Instability index: 55.68 | ||
Aliphatic index: 95.49 | Aliphatic index: 95.49 | ||
Grand Average of Hydropathicity (GRAVY): -0.311 | Grand Average of Hydropathicity (GRAVY): -0.311 | ||
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Alpha-helices: 34.78% | Alpha-helices: 34.78% | ||
Extended (Beta-sheets): 20.65% | Extended (Beta-sheets): 20.65% | ||
Other (Loops): 44.57% | Other (Loops): 44.57% | ||
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== The Full-Length '' R. sphaeroides '' PrrA Amino Acid Sequence | == The Full-Length '' R. sphaeroides '' PrrA Amino Acid Sequence (184 Residues) == | ||
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1 maedlvfelg adrslllvdd depflkrlak amekrgfvle taqsvaegka iaqarppaya | 1 maedlvfelg adrslllvdd depflkrlak amekrgfvle taqsvaegka iaqarppaya | ||
61 vvdlrledgn gldvvevlre rrpdcrivvl tgygaiatav aavkigatdy lskpadanev | 61 vvdlrledgn gldvvevlre rrpdcrivvl tgygaiatav aavkigatdy lskpadanev | ||
121 thallakges lppppenpms adrvrwehiq riyemcdrnv setarrlnmh rrtlqrilak | 121 thallakges lppppenpms adrvrwehiq riyemcdrnv setarrlnmh rrtlqrilak | ||
181 rspr | 181 rspr | ||