User:Yana Fedotova/Sandbox 1: Difference between revisions

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== Background Information ==
== Background Information ==
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PrrA is a DNA binding protein and a essential part of the two-component signal transduction regulatory system, PrrBA.  ''Rhodobacter capsulatus'' contains RegA considered a homologue to PrrA protein in ''R. sphaeroides''.  PrrA is proposed to be a master regulator/response regulator involved in oxygen regulation of photosynthesis genes expression responding to changes to the rate of electron flow as means to maintain redox balance, such as a balance between energy production and energy consumption in the cell. <ref> Zeilstra-Ryalls, J. H. and S. Kaplan.  2004.  Oxygen Intervention in the Regulation of Gene Expression: The Photosynthetic Bacterial Paradigm.  Cellular and Molecular Life Sciences 61:417-436.</ref>.  PrrA is thought to play an important function in carbon dioxide and nitrogen fixations, photosynthesis as well as proton oxidation and uptake. <ref> Eraso, J. M., J. H. Roh, X. Zeng, S. J. Callister, M. S. Lipton and S. Kaplan.  2008. Role of Global Transcriptional Regulator PrrA in ''Rhodobacter sphaeroides'' 2.4.1: Combined Transcriptome and Proteome Analysis.  Journal of Bacteriology 190: 4831-4848.</ref>.   
PrrA is a DNA binding protein and a essential part of the two-component signal transduction regulatory system, PrrBA.  ''Rhodobacter capsulatus'' contains RegA considered a homologue to PrrA protein in ''R. sphaeroides''.  PrrA is proposed to be a master regulator/response regulator involved in oxygen regulation of photosynthesis genes expression responding to changes to the rate of electron flow as means to maintain redox balance, such as a balance between energy production and energy consumption in the cell  <ref> Zeilstra-Ryalls, J. H. and S. Kaplan.  2004.  Oxygen Intervention in the Regulation of Gene Expression: The Photosynthetic Bacterial Paradigm.  Cellular and Molecular Life Sciences 61:417-436.</ref>.  PrrA is thought to play an important function in carbon dioxide and nitrogen fixations, photosynthesis as well as proton oxidation and uptake  <ref> Eraso, J. M., J. H. Roh, X. Zeng, S. J. Callister, M. S. Lipton and S. Kaplan.  2008. Role of Global Transcriptional Regulator PrrA in ''Rhodobacter sphaeroides'' 2.4.1: Combined Transcriptome and Proteome Analysis.  Journal of Bacteriology 190: 4831-4848.</ref>.   
PrrB is considered a sensor histidine kinase bound to the cytoplasmic membrane that can phosphorylate PrrA when it senses changes the aforementioned changes in the cell.  Interestingly, “1,058 genes were proposed to be regulated out of 4,284 genes as represented on the GeneChip of ''R. sphaeroides'' 2.4.1” by PrrA. <ref> Eraso, J. M. and S. Kaplan.  1994.  ''prrA'', a Putative Response Regulator Involved in Oxygen Regulation of Photosynthesis Gene Expression in ''Rhodobacter sphaeroides''.  Journal of Bacteriology 176:32-43.</ref>.
PrrB is considered a sensor histidine kinase bound to the cytoplasmic membrane that can phosphorylate PrrA when it senses changes the aforementioned changes in the cell.  Interestingly, “1,058 genes were proposed to be regulated out of 4,284 genes as represented on the GeneChip of ''R. sphaeroides'' 2.4.1” by PrrA  <ref> Eraso, J. M. and S. Kaplan.  1994.  ''prrA'', a Putative Response Regulator Involved in Oxygen Regulation of Photosynthesis Gene Expression in ''Rhodobacter sphaeroides''.  Journal of Bacteriology 176:32-43.</ref>.
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== Structure of the Proposed PrrA Protein ==
== Structure of the Proposed PrrA Protein ==
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<references/>
<references/>
1. Eraso, J. M., J. H. Roh, X. Zeng, S. J. Callister, M. S. Lipton and S. Kaplan. 2008.  Role of Global Transcriptional Regulator PrrA in ''Rhodobacter sphaeroides'' 2.4.1: Combined Transcriptome and Proteome Analysis.  Journal of Bacteriology 190: 4831-4848.
   
 
2. Eraso, J. M. and S. Kaplan. 1994. prrA, a Putative Response Regulator Involved in Oxygen Regulation of Photosynthesis Gene Expression in ''Rhodobacter sphaeroides''. Journal of Bacteriology 176:32-43.
 
3. Laguri, C., M. K. Phillips-Jones, and M. P. Williamson. 2003. Solution Structure and DNA Binding of the Effector Domain from the Global Regulator PrrA (RegA) from ''Rhodobacter sphaeroides'': Insights into DNA Binding Specificity. Nucleic Acids Research 31:6778-6787.
 
4. Zeilstra-Ryalls, J. H. and S. Kaplan. 2004. Oxygen intervention in the regulation of gene expression: the photosynthetic bacterial paradigm. Cellular and Molecular Life Sciences 61:417-436.


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