User:Susana Retamal/Sandbox1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically diverse bacterial species. The bacterium ''Rhodobacter sphaeroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically diverse bacterial species. The bacterium ''Rhodobacter sphaeroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | ||
''Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw''' | =''Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw'''= | ||
<scene name='User:Susana_Retamal/Sandbox1/Rsp1275/8'>TextToBeDisplayed</scene> | <scene name='User:Susana_Retamal/Sandbox1/Rsp1275/8'>TextToBeDisplayed</scene> | ||
=Protein Sequence= | =Protein Sequence= | ||
Revision as of 10:25, 1 May 2009
Background InformationBackground Information
Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from E. coli have been identified in a variety of taxonomically diverse bacterial species. The bacterium Rhodobacter sphaeroides 2.4.1 encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in Rhodobacter spheroides. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes.
Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw'Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw'
Protein SequenceProtein Sequence
mfvpapdati tncrncplrr kplflpfsds elsfmeqfkv gelvvapgvt lleegqgsah lftvlsglgi rstmlengrr qvinflfpgd figlqaglag emrhsvestt tmvlcvfnra dlwdlfreep eraydltwia aveehflget iaslgqrdat erlawallri herlsaigla ergrvpmpwr qqdladalgl slvhtnktir rlretghalw eggtlfvdre rlatlaladp drprrrpli
Evidence of Fnr-Crp protein Involvement in Transcriptional Response to NaClEvidence of Fnr-Crp protein Involvement in Transcriptional Response to NaCl
It has been proposed that Fnr-Crp protein encoded by rsp1275 is a transcription factor responsible for salt-responsive changes in the transcriptome. In addition, it's likely that rsp1275 interacts with a small ligand (potentially Na or Cl).
Physico - Chemical parameters for Rsp1275Physico - Chemical parameters for Rsp1275
from http://ca.expasy.org/tools/protparam.html
Number of amino acids: 249
Molecular weight: 28014.2
Theoretical pI: 6.10
Atomic composition:
Carbon C 1249 Hydrogen H 1986 Nitrogen N 360 Oxygen O 355 Sulfur S 9
Formula: C1249H1986N360O355S9 Total number of atoms: 3959
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
- Ext. coefficient 29115
Abs 0.1% (=1 g/l) 1.039, assuming ALL Cys residues appear as half cystines
- Ext. coefficient 28990
Abs 0.1% (=1 g/l) 1.035, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is:
- 30 hours (mammalian reticulocytes, in vitro).
- >20 hours (yeast, in vivo).
- >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.06 This classifies the protein as unstable.
Aliphatic index: 97.19
Grand average of hydropathicity (GRAVY): -0.097
Globe Prediction: it appears as compact, as a globular domain.
Secondary Structure: Helix 34.94% Extended (sheet) 17.67% Loop 47.39%
| Amino Acid | Number present | Percentage of total present |
|---|---|---|
| Ala (A) | 15 | 8.8% |
| Arg (R) | 10 | 10.4% |
| Asn (N) | 6 | 2.4% |
| Asp (D) | 10 | 4.8% |
| Cys (C) | 1 | 1.2% |
| Gln (Q) | 5 | 2.8% |
| Glu (E) | 11 | 8.0% |
| Gly (G) | 9 | 7.6% |
| His (H) | 10 | 2.4% |
| Ile (I) | 3 | 4.0% |
| Leu (L) | 21 | 14.5% |
| Lys (K) | 6 | 1.2% |
| Met (M) | 6 | 2.4% |
| Phe (F) | 2 | 4.0% |
| Pro (P) | 8 | 5.2% |
| Ser (S) | 7 | 4.4% |
| Thr (T) | 13 | 6.4% |
| Trp (W) | 2 | 2.0% |
| Tyr (Y) | 6 | 0.4% |
| Val (V) | 12 | 5.6% |
| Pyl (O) | 0 | 0.% |
| Sec (U) | 0 | 0.0% |
Amino Acid Conservation ScoresAmino Acid Conservation Scores
- POS: The position of the AA in the SEQRES derived sequence. - SEQ: The SEQRES derived sequence in one letter code. - 3LATOM: The ATOM derived sequence in three letter code, including the AA's positions as they appear in the PDB file and the chain identifier. - SCORE: The normalized conservation scores. - COLOR: The color scale representing the conservation scores (9 - conserved, 1 - variable). - CONFIDENCE INTERVAL: When using the bayesian method for calculating rates, a confidence interval is assigned to each of the inferred evolutionary conservation scores. - CONFIDENCE INTERVAL COLORS: When using the bayesian method for calculating rates. The color scale representing the lower and upper bounds of the confidence interval. - MSA DATA: The number of aligned sequences having an amino acid (non-gapped) from the overall number of sequences at each position. - RESIDUE VARIETY: The residues variety at each position of the multiple sequence alignment.
POS SEQ 3LATOM SCORE COLOR CONFIDENCE INTERVAL CONFIDENCE INTERVAL COLORS MSA DATA RESIDUE VARIETY (normalized) 1 P PRO47: -1.063 8* -1.567,-0.697 9,7 3/19 P 2 G GLY48: -0.916 8 -1.417,-0.529 9,7 13/19 G,K 3 V VAL49: 1.226 2 0.404, 2.816 4,1 13/19 C,E,Q,R,V 4 T THR50: 1.816 1 0.790, 2.816 3,1 14/19 A,E,Q,T 5 L LEU51: 0.649 3* -0.136, 1.367 5,1 18/19 I,L,V 6 L LEU52: -0.142 5 -0.697, 0.404 7,4 18/19 F,I,L,Y 7 E GLU53: 1.061 2* 0.108, 1.367 5,1 18/19 A,E,I,K,Q,S,W 8 E GLU54: 0.909 2* 0.108, 1.367 5,1 18/19 A,E,Q,S 9 G GLY55: -0.349 6 -0.852, 0.108 7,5 18/19 D,E,G,P 10 Q GLN56: -0.600 7 -0.998,-0.344 8,6 18/19 D,E,Q,S 11 G GLY57: 2.391 1 2.816, 2.816 1,1 18/19 E,G,I,K,L,P,S,T,V 12 S SER58: 0.521 4* -0.136, 1.367 5,1 18/19 A,L,M,N,S,T 13 A ALA59: 2.175 1 1.367, 2.816 1,1 18/19 A,D,K,N,R,S,T 14 H HIS60: -0.278 6 -0.852, 0.108 7,5 18/19 F,H,K,S 15 L LEU61: 1.898 1 0.790, 2.816 3,1 18/19 C,F,I,L,V,Y 16 F PHE62: 0.468 4 -0.136, 0.790 5,3 18/19 A,F,L,S,Y 17 T THR63: 0.156 5* -0.529, 0.790 7,3 18/19 A,N,Q,S,T,V 18 V VAL64: -0.901 8 -1.277,-0.697 9,7 18/19 I,L,V 19 L LEU65: 0.975 2* 0.108, 1.367 5,1 18/19 A,I,L,M,R,S,V 20 S SER66: -0.045 5 -0.697, 0.404 7,4 18/19 E,H,R,S,T 21 G GLY67: -1.418 9 -1.755,-1.277 9,9 18/19 G 22 L LEU68: 0.334 4 -0.344, 0.790 6,3 18/19 A,I,K,L,T,V 23 G GLY69: 0.572 3* -0.136, 1.367 5,1 18/19 A,G,I,L,M,V 24 I ILE70: -0.318 6 -0.852, 0.108 7,5 18/19 I,K,R,T 25 R ARG71: 0.405 4 -0.344, 0.790 6,3 18/19 I,L,R,S,T 26 S SER72: -0.248 6 -0.852, 0.108 7,5 18/19 C,S,T,Y 27 T THR73: -0.039 5 -0.529, 0.404 7,4 18/19 K,Q,R,T 28 M MET74: -0.296 6 -0.852, 0.108 7,5 19/19 I,L,M,T,V 29 L LEU75: -0.075 5 -0.529, 0.404 7,4 19/19 A,L,M,S,T 30 E GLU76: 0.959 2* 0.108, 1.367 5,1 19/19 E,G,P,S,T 31 N ASN77: 0.131 5 -0.344, 0.404 6,4 19/19 D,E,N,Q,S,T 32 G GLY78: -1.441 9 -1.755,-1.277 9,9 19/19 G 33 R ARG79: -0.815 7 -1.139,-0.529 8,7 19/19 D,E,K,R 34 R ARG80: -0.887 8 -1.277,-0.529 9,7 19/19 E,Q,R 35 Q GLN81: -1.577 9 -1.755,-1.417 9,9 19/19 Q 36 V VAL82: -0.767 7 -1.139,-0.529 8,7 19/19 I,L,M,V 37 I ILE83: -0.369 6 -0.852, 0.108 7,5 19/19 E,I,L,T,V 38 N ASN84: 0.834 3* 0.108, 1.367 5,1 19/19 A,G,N,R,S 39 F PHE85: -0.749 7 -1.139,-0.344 8,6 19/19 F,L,V 40 L LEU86: 0.791 3* 0.108, 1.367 5,1 19/19 A,C,D,H,L,Q 41 F PHE87: -0.246 6 -0.852, 0.108 7,5 19/19 F,G,L 42 P PRO88: -0.534 7 -0.998,-0.136 8,5 19/19 A,P,V 43 G GLY89: -0.907 8 -1.277,-0.697 9,7 19/19 G,P,S 44 D ASP90: -1.388 9 -1.567,-1.277 9,9 19/19 D,E 45 F PHE91: 0.303 4 -0.344, 0.790 6,3 19/19 A,F,L,M,V,Y 46 I ILE92: -0.023 5 -0.697, 0.404 7,4 19/19 E,F,I,L,V 47 G GLY93: -1.441 9 -1.755,-1.277 9,9 19/19 G 48 L LEU94: 0.935 2* 0.108, 1.367 5,1 19/19 E,F,L,M,R,W 49 Q GLN95: 0.552 3* -0.136, 1.367 5,1 19/19 A,D,E,N,P,Q 50 A ALA96: -0.391 6 -1.139, 0.108 8,5 13/19 A,G 51 G GLY97: 0.022 5 -0.697, 0.404 7,4 13/19 G,I,L 52 L LEU98: 2.561 1 2.816, 2.816 1,1 19/19 A,F,G,H,L,M,P,S,T 53 A ALA99: 2.362 1 1.367, 2.816 1,1 19/19 A,G,N,Q,R,S,T,V 54 G GLY100: 2.565 1 2.816, 2.816 1,1 19/19 D,E,G,I,L,M,N,Q,R,S 55 E GLU101: 1.976 1 1.367, 2.816 1,1 19/19 A,E,H,K,N,Q,R,S,T 56 M MET102: 0.254 4 -0.344, 0.790 6,3 19/19 H,M,N,S,V,Y 57 R ARG103: 1.111 2 0.404, 1.367 4,1 19/19 A,N,P,Q,R,S,T,V 58 H HIS104: 0.562 3* -0.136, 1.367 5,1 19/19 F,G,H,L,N,S,Y 59 S SER105: -0.561 7 -0.998,-0.136 8,5 19/19 D,F,S,Y 60 V VAL106: -1.054 8 -1.417,-0.852 9,7 19/19 A,T,V 61 E GLU107: -0.753 7 -1.139,-0.529 8,7 19/19 D,E,Q,T 62 S SER108: -1.062 8 -1.417,-0.852 9,7 19/19 A,S,T 63 T THR109: -0.040 5 -0.529, 0.404 7,4 19/19 A,I,L,T,V 64 T THR110: -0.845 7 -1.277,-0.529 9,7 19/19 E,G,S,T 65 T THR111: 0.081 5* -0.529, 0.790 7,3 19/19 A,D,E,G,T 66 M MET112: 0.816 3* 0.108, 1.367 5,1 19/19 A,C,L,M,S,T,V 67 V VAL113: 0.008 5 -0.529, 0.404 7,4 19/19 E,L,M,R,T,V 68 L LEU114: -0.103 5 -0.697, 0.404 7,4 19/19 I,L,M,V 69 C CYS115: -0.836 7 -1.277,-0.529 9,7 19/19 A,C,V 70 V VAL116: 2.036 1 1.367, 2.816 1,1 19/19 C,E,I,K,Q,R,T,V 71 F PHE117: -0.769 7 -1.277,-0.344 9,6 19/19 F,I,V 72 N ASN118: 1.494 1 0.790, 2.816 3,1 19/19 A,F,G,K,N,P,R,S 73 R ARG119: -0.223 6 -0.697, 0.108 7,5 19/19 A,F,K,R,Y 74 A ALA120: 1.689 1 0.790, 2.816 3,1 18/19 A,D,E,G,K,N,S,T 75 D ASP121: 1.492 1 0.790, 2.816 3,1 18/19 D,I,L,P,T,V 76 L LEU122: -0.309 6 -0.852, 0.108 7,5 18/19 F,L 77 W TRP123: 1.635 1 0.790, 2.816 3,1 18/19 D,E,F,G,L,Q,W 78 D ASP124: 0.991 2* 0.108, 1.367 5,1 18/19 A,D,E,Q,R 79 L LEU125: -0.503 6 -0.998,-0.136 8,5 18/19 F,L,M,R 80 F PHE126: 0.962 2* 0.108, 1.367 5,1 18/19 A,F,I,L,M,S 81 R ARG127: 1.061 2* 0.108, 1.367 5,1 19/19 E,G,L,Q,R,S 82 E GLU128: 0.612 3* -0.136, 1.367 5,1 19/19 E,K,N,S,T 83 E GLU129: 0.413 4 -0.344, 0.790 6,3 19/19 D,E,M,Q,R,T 84 P PRO130: -1.243 9 -1.567,-0.998 9,8 19/19 P,Q 85 E GLU131: -0.080 5 -0.697, 0.404 7,4 19/19 E,H,K,N,Q 86 R ARG132: 0.801 3* 0.108, 1.367 5,1 19/19 I,L,M,R,V 87 A ALA133: 0.460 4 -0.344, 0.790 6,3 19/19 A,G,L,Q,R,S 88 Y TYR134: 0.879 2* 0.108, 1.367 5,1 19/19 C,H,L,Q,R,Y 89 D ASP135: 0.376 4 -0.344, 0.790 6,3 19/19 A,D,E,Q,R,S 90 L LEU136: -0.372 6 -0.852, 0.108 7,5 19/19 I,L,M 91 T THR137: 0.789 3* 0.108, 1.367 5,1 19/19 H,L,M,N,T,W 92 W TRP138: 0.918 2* 0.108, 1.367 5,1 19/19 A,D,E,R,T,W 93 I ILE139: 0.435 4 -0.344, 0.790 6,3 19/19 E,F,I,L,M,Q 94 A ALA140: -0.562 7 -0.998,-0.136 8,5 19/19 A,M,N,S,T 95 A ALA141: 0.604 3* -0.136, 1.367 5,1 19/19 A,F,I,L,S,T 96 V VAL142: 1.537 1 0.790, 2.816 3,1 19/19 A,D,G,K,N,R,S,T,V 97 E GLU143: -1.054 8 -1.417,-0.852 9,7 19/19 E,G,K 98 E GLU144: -0.200 6 -0.697, 0.108 7,5 19/19 E,I,L,M 99 H HIS145: 0.536 3* -0.136, 1.367 5,1 19/19 A,D,H,K,M,Q,S 100 F PHE146: 1.103 2* 0.108, 2.816 5,1 19/19 A,F,G,L,N,Q,R,S 101 L LEU147: -0.661 7 -1.139,-0.344 8,6 19/19 A,D,L 102 G GLY148: -0.441 6 -0.852,-0.136 7,5 19/19 E,G,Q,R 103 E GLU149: -0.539 7 -0.998,-0.136 8,5 19/19 D,E,K,Q 104 T THR150: 0.063 5 -0.529, 0.404 7,4 19/19 H,M,Q,T,W 105 I ILE151: -0.555 7 -0.998,-0.136 8,5 19/19 A,I,L,M 106 A ALA152: -0.041 5 -0.697, 0.404 7,4 19/19 A,G,L,V 107 S SER153: 0.364 4 -0.344, 0.790 6,3 19/19 L,S,T,V,Y 108 L LEU154: -1.173 8 -1.567,-0.998 9,8 19/19 I,L 109 G GLY155: -1.243 9 -1.567,-0.998 9,8 19/19 G,S 110 Q GLN156: -0.805 7 -1.139,-0.529 8,7 19/19 K,N,Q,R 111 R ARG157: 0.138 5 -0.344, 0.404 6,4 19/19 D,K,M,Q,R 112 D ASP158: -0.224 6 -0.697, 0.108 7,5 19/19 C,D,N,S,T 113 A ALA159: -1.362 9 -1.567,-1.139 9,8 19/19 A,I 114 T THR160: 0.916 2* 0.108, 1.367 5,1 19/19 D,E,N,Q,R,T,V 115 E GLU161: -1.294 9 -1.567,-1.139 9,8 19/19 E,V 116 R ARG162: -0.855 7 -1.277,-0.529 9,7 19/19 K,R 117 L LEU163: -0.418 6 -0.852,-0.136 7,5 19/19 I,L,V 118 A ALA164: -1.261 9 -1.567,-1.139 9,8 19/19 A,G,T 119 W TRP165: -0.387 6 -0.852,-0.136 7,5 19/19 A,H,S,W 120 A ALA166: -0.327 6 -0.852, 0.108 7,5 19/19 A,F,L,Y 121 L LEU167: -0.623 7 -1.139,-0.344 8,6 19/19 I,L 122 L LEU168: 2.061 1 1.367, 2.816 1,1 19/19 A,H,L,S,V,Y 123 R ARG169: 0.245 4 -0.344, 0.790 6,3 19/19 D,G,I,L,N,R 124 I ILE170: -0.525 6 -0.998,-0.136 8,5 19/19 I,L,W 125 H HIS171: 0.296 4 -0.344, 0.790 6,3 19/19 A,C,H,R,S 126 E GLU172: 2.674 1 2.816, 2.816 1,1 19/19 C,D,E,H,K,N,Q,R,T 127 R ARG173: -1.085 8 -1.417,-0.852 9,7 19/19 A,H,R 128 L LEU174: 1.821 1 0.790, 2.816 3,1 19/19 A,D,F,L,Q,S,Y 129 S SER175: 1.545 1 0.790, 2.816 3,1 19/19 A,E,G,L,S 130 A ALA176: 0.878 2* -0.136, 1.367 5,1 19/19 A,G,N,P,Q,R,T 131 I ILE177: 1.408 1 0.404, 2.816 4,1 17/19 E,I,K,P,Q,R,T 132 G GLY178: -0.288 6 -0.852, 0.108 7,5 17/19 G,N,S,T 133 L LEU179: 0.548 3* -0.344, 1.367 6,1 17/19 A,F,L,N,V 134 A ALA180: -0.547 7 -0.998,-0.136 8,5 17/19 A,G,S,T 135 E GLU181: 1.841 1 0.790, 2.816 3,1 18/19 A,D,E,G,H,K,P,S 136 R ARG182: 0.783 3* -0.136, 1.367 5,1 17/19 D,K,M,R,T,V 137 G GLY183: 0.172 5* -0.697, 0.790 7,3 2/19 G,T 138 R ARG184: 0.226 4* -0.529, 0.790 7,3 19/19 A,E,Q,R,T 139 V VAL185: -0.496 6 -1.139,-0.136 8,5 19/19 F,L,V 140 P PRO186: 0.687 3* -0.136, 1.367 5,1 19/19 D,P,R,S,T 141 M MET187: -0.889 8 -1.277,-0.529 9,7 19/19 L,M,V 142 P PRO188: -0.993 8 -1.417,-0.697 9,7 19/19 A,P,T 143 W TRP189: -1.104 8 -1.417,-0.852 9,7 19/19 L,M,W 144 R ARG190: -0.591 7 -0.998,-0.344 8,6 19/19 A,G,R,S,T 145 Q GLN191: -1.107 8 -1.417,-0.852 9,7 19/19 Q,R,W 146 Q GLN192: -0.300 6 -0.852, 0.108 7,5 19/19 A,E,G,Q,T 147 D ASP193: -0.990 8 -1.277,-0.697 9,7 19/19 D,E,Q 148 L LEU194: -0.925 8 -1.277,-0.697 9,7 19/19 I,L,M 149 A ALA195: -1.427 9 -1.755,-1.277 9,9 19/19 A,G 150 D ASP196: -1.403 9 -1.567,-1.277 9,9 19/19 D,N 151 A ALA197: 0.072 5 -0.529, 0.404 7,4 19/19 A,F,Y 152 L LEU198: -1.443 9 -1.755,-1.277 9,9 19/19 L 153 G GLY199: -1.441 9 -1.755,-1.277 9,9 19/19 G 154 L LEU200: -1.044 8 -1.417,-0.697 9,7 19/19 L,T 155 S SER201: -1.493 9 -1.755,-1.417 9,9 19/19 S,T 156 L LEU202: -0.226 6 -0.697, 0.108 7,5 19/19 I,L,P,V 157 V VAL203: -1.296 9 -1.567,-1.139 9,8 19/19 E,V 158 H HIS204: -1.435 9 -1.755,-1.277 9,9 19/19 H,T 159 T THR205: -1.190 8 -1.417,-0.998 9,8 19/19 I,T,V 160 N ASN206: -1.519 9 -1.755,-1.417 9,9 19/19 N,S 161 K LYS207: -1.390 9 -1.567,-1.277 9,9 19/19 K,R 162 T THR208: -0.802 7 -1.277,-0.529 9,7 19/19 L,Q,T,V 163 I ILE209: 0.127 5* -0.529, 0.790 7,3 19/19 F,I,L,M,V 164 R ARG210: -0.459 6 -0.852,-0.136 7,5 19/19 G,K,R,S,T 165 R ARG211: -0.501 6 -0.998,-0.136 8,5 19/19 A,K,R,Y 166 L LEU212: -0.866 7 -1.277,-0.529 9,7 19/19 F,L 167 R ARG213: 0.305 4 -0.344, 0.790 6,3 18/19 A,E,K,Q,R 168 E GLU214: -0.134 5 -0.697, 0.404 7,4 18/19 E,K,R 169 T THR215: 1.641 1 0.790, 2.816 3,1 16/19 D,H,I,L,M,Q,S,T 170 G GLY216: -1.323 9 -1.755,-1.139 9,8 14/19 G 171 H HIS217: 0.687 3* -0.136, 1.367 5,1 4/19 A,H,M,V 172 A ALA218: -0.749 7* -1.277,-0.344 9,6 4/19 A,I 173 L LEU219: -0.200 6* -0.852, 0.404 7,4 4/19 A,L,T 174 W TRP220: 0.478 4* -0.344, 1.367 6,1 4/19 I,L,V,W 175 E GLU221: 0.163 5* -0.697, 0.790 7,3 4/19 E,I,Q 176 G GLY222: -0.526 7* -1.139,-0.136 8,5 4/19 G,H 177 G GLY223: -0.842 7* -1.417,-0.529 9,7 2/19 G 178 T THR224: 0.181 4* -0.697, 0.790 7,3 2/19 G,T 179 L LEU225: -0.327 6* -1.139, 0.404 8,4 1/19 L 180 F PHE226: -0.327 6* -1.139, 0.404 8,4 1/19 F 181 V VAL227: -0.327 6* -1.139, 0.404 8,4 1/19 V
Phylogenetic Tree of rsp1275Phylogenetic Tree of rsp1275
http://consurf.tau.ac.il/results/1240240806/treeView.html
ReferencesReferences
- Spiro, S. 1994. The FNR family of transcriptional regulators. Antonie van Leeuwenhoek 66:23–36.
- PredictProtein: B Rost, G Yachdav and J Liu (2004). The PredictProtein Server. Nucleic Acids Research 32(Web Server issue):W321-W326.
Page Created by Susana Retamal, email:susana.retamal@gmail.com
Acknownledment: Jill Zeilstra Ryalls, Adam Meade and Yana Fedotova.