User:Susana Retamal/Sandbox1: Difference between revisions
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Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically diverse bacterial species. The bacterium ''Rhodobacter sphaeroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically diverse bacterial species. The bacterium ''Rhodobacter sphaeroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes. | ||
''Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw'''<applet load='rsp1275.pdb' size='300' color=' | ''Structure of the Proposed rsp1275 modeling obtained using 3D-Jigsaw''' | ||
<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D-Jigsaw Image of proposed rsp1275 protein'/> | |||
<scene name='User:Susana_Retamal/Sandbox_1/Secondary_structure_-_1275/1'>Secondary Structure</scene> | |||
{{Template:ColorKey_Amino2CarboxyRainbow}} | |||
=Protein Sequence= | =Protein Sequence= | ||