User:Susana Retamal/Sandbox1: Difference between revisions

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=Background Information=
=Background Information=


Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically (1) diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes.  
Rsp1275 is a member of the Fnr-Crp family of transcriptional regulators. Homologues of the Fnr protein from ''E. coli'' have been identified in a variety of taxonomically <1> diverse bacterial species. The bacterium ''Rhodobacter spheroides 2.4.1'' encodes 8 members of this family, however only 2 have known function. The modeling of 7 of them were successfully obtained using 3D-Jigsaw, all being very similar in structure to Crp (the only one with crystal structure solved). The DNA binding domain helix-turn-helix is located in the C-terminal, and the N-terminal part contains the allosteric effector domain. Rsp1275 has been linked with salt-tolerance in ''Rhodobacter spheroides''. Different genes coding for the Fnr-Crp proteins of unknown function have been cloned and a multicopy test was performed to evaluate these as potential regulators of NaCl-responsive genes.  


''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/>
''Structure of the Proposed rsp1275'''<applet load='rsp1275.pdb' size='300' color='black' frame='true' align='right' caption='3D Image of proposed rsp1275 protein'/>
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=Evidence of Fnr-Crp protein Involvement in Transcriptional       Response to NaCl=
=Evidence of Fnr-Crp protein Involvement in Transcriptional Response to NaCl=




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Theoretical pI: 6.10
Theoretical pI: 6.10


Globe Prediction: it appears as compact, as a globular doimain.
Secondary Structure:
          Helix 34.94%
          Extended (sheet) 17.67%
          Loop 47.39%


{| border="1"
{| border="1"
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=References=
=References=


*Spiro, S. 1994. The FNR family of transcriptional regulators. Antonie van Leeuwenhoek 66:23–36.
<1>Spiro, S. 1994. The FNR family of transcriptional regulators. Antonie van Leeuwenhoek 66:23–36.
*PredictProtein: B Rost, G Yachdav and J Liu (2004) The PredictProtein Server. Nucleic Acids Research 32(Web Server issue):W321-W326.
<2>PredictProtein: B Rost, G Yachdav and J Liu (2004) The PredictProtein Server. Nucleic Acids Research 32(Web Server issue):W321-W326.
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