User:Nathan Roy: Difference between revisions

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The HIV-1 Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell, and contains four functional segments; MA, CA (NTD and CTD), NC, and p6. The NC region is flanked by two "spacer" segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains. Gag is often referred to as an "assembly machine", because expression of Gag alone is sufficient to produce budding virus-like particles (VLP's), due to multimerization of roughly 2000 Gag molecules per virion. Here, we will take a closer look at the MA and CA domains, and how the structural components of these domains aid in the assembly of virus particles. <applet load='2H3F_mono1.pdb' size='300' frame='true' align='right' caption=' FIGURE 1. MA unbound to PI(4,5)P2' scene=''/>

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	The HIV-1 Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell, and contains four functional segments; MA, CA (NTD and CTD), NC, and p6. The NC region is flanked by two "spacer" segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains. Gag is often referred to as an "assembly machine", because expression of Gag alone is sufficient to produce budding virus-like particles (VLP's), due to multimerization of roughly 2000 Gag molecules per virion. Here, we will take a closer look at the MA and CA domains, and how the structural components of these domains aid in the assembly of virus particles. <applet load='2H3F_mono1.pdb' size='300' frame='true' align='right' caption=' FIGURE 1. MA unbound to PI(4,5)P2' scene=''/>		The HIV-1 Gag protein is the major structural protein required for virus assembly. It is synthesized as a polyprotein in the cytosol of an infected cell, and contains four functional segments; MA, CA (NTD and CTD), NC, and p6. The NC region is flanked by two "spacer" segments, denoted SP1 and SP2. The polyprotein is all alpha helical, except the NC region, which is composed of two RNA interacting zinc knuckle domains. Gag is often referred to as an "assembly machine", because expression of Gag alone is sufficient to produce budding virus-like particles (VLP's), due to multimerization of roughly 2000 Gag molecules per virion. Here, we will take a closer look at the MA and CA domains, and how the structural components of these domains aid in the assembly of virus particles. <applet load='2H3F_mono1.pdb' size='300' frame='true' align='right' caption=' FIGURE 1. MA unbound to PI(4,5)P2' scene=''/>

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