Sandbox Eric Martz: Difference between revisions
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Proposed article title: <big><b>Release of | Proposed article title: <big><b>Release of | ||
RING | |||
from | from | ||
Cullin | |||
by | by | ||
NEDD8ylation</b></big> | |||
<applet load='3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='NEDD8ylation of Cullin-RING' | <applet load='3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='NEDD8ylation of Cullin-RING' | ||
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</jmol> | </jmol> | ||
<b><font color='#a04090'>RING (A)</font> | |||
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<font color='#b0a090'>Cullin (C)</font> | |||
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<font color='#a03000'>NEDD8 (A)</font></b> | |||
Display chains as smoothed backbone traces colored by N->C Rainbow: | Display chains as smoothed backbone traces colored by N->C Rainbow: | ||
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<font color='#b0a090'>Cullin (C)</font> | <font color='#b0a090'>Cullin (C)</font> | ||
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<font color='#a03000'>NEDD8</font></b> | <font color='#a03000'>NEDD8 (A)</font></b> | ||
</center> | </center> | ||
Revision as of 21:59, 22 February 2009
This article is under collaborative development by Eric Martz (UMass) and Raymond J. Deshaies and his team (CalTech). Please do not edit this page unless you are a member of the development team. Once completed, this page may be moved to a permanent article title.
Proposed article title: Release of RING from Cullin by NEDD8ylation
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RING (A) Cullin (C) NEDD8 (A)
Display chains as smoothed backbone traces colored by N->C Rainbow:
| N | C |
Color traces by chain:
RING (A) Cullin (C) NEDD8 (A)
Contacts to:
The morph [1] button above shows the transition from a Cullin-RING ligase dimer (3dpl) to the trimer resulting from NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to Lys724 of Cullin; 3dqv). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by the RING N-terminus which remains embedded in Cullin. The contraction and re-expansion of the C-terminal domain during rotation is an artifact of the morphing algorithm.
Notes and ReferencesNotes and References
- ↑ The 9-model morph file can be downloaded from File:3dpl 3dqv acr morph.pdb. The morph represents the change from 3dpl to 3dqv, and only chains C and R which are common to these two models. Only one trimer was used from 3dqv (which contains 2 trimers in the asymmetric unit), namely, the one with chains named A, C, and R. This trimer was obtained from PQS as 3dqv_1.mmol. The morph was performed by the Yale Morph Server (beta server), ID b899128-21508. This produced 8 models numbered 0-7. In the product morph, the orientation of the final chains was not changed. Hence, chain A (NEDD8) from 3dqv.pdb was added as model number 8 (9th model) without the need for special alignment. Morph done by Eric Martz at the request of Raymond J. Deshaies of CalTech.