Group:SMART:Tangible Models of Cdc42 Interacting With Intersectin: Difference between revisions
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Cdc42 with GDP |
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='''<font color = 'black'>Cdc42: The GTPase</font>'''= | ='''<font color = 'black'>Cdc42: The GTPase</font>'''= | ||
Like other Rho GTPases, Cdc42 can exist in either the “Off” or “On” state. A guanine diphosphate (GDP) in the nucleotide-binding pocket indicates the “Off” state, and a guanine triphosphate (GTP) indicates the “On” state. _e activation from the “Off” state to the “On” state is catalyzed by a guanine-nucleotide exchange factor (GEF). Thee hydrolysis of GTP, which turns the GTPase off, is catalyzed by a GTPase activating protein called a GAP. | Like other Rho GTPases, <scene name='SMART_Lincoln_2009/Cdc42_and_gdp/1'>Cdc42</scene> can exist in either the “Off” or “On” state. A guanine diphosphate (GDP) in the nucleotide-binding pocket indicates the “Off” state, and a guanine triphosphate (GTP) indicates the “On” state. _e activation from the “Off” state to the “On” state is catalyzed by a guanine-nucleotide exchange factor (GEF). Thee hydrolysis of GTP, which turns the GTPase off, is catalyzed by a GTPase activating protein called a GAP. | ||
In addition to binding to a nucleotide (GDP or GTP), Cdc42 is usually found to be bound to a magnesium ion (Mg++), shown in magenta. The Mg++ sits near the nucleotide-binding pocket and helps to stabilize the Cdc42-nucleotide interaction. | In addition to binding to a nucleotide (GDP or GTP), Cdc42 is usually found to be bound to a magnesium ion (Mg++), shown in magenta. The Mg++ sits near the nucleotide-binding pocket and helps to stabilize the Cdc42-nucleotide interaction. | ||
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='''<font color = 'black'>How Intersectin Activates Cdc42</font>'''= | ='''<font color = 'black'>How Intersectin Activates Cdc42</font>'''= | ||
<applet load='2rh1.pdb' size=' | <applet load='2rh1.pdb' size='350' frame='true' align='left' scene='SMART_Lincoln_2009/Complex_intersectin_and_cdc42/1'/> | ||
Cdc42/Intersectin Complex | <scene name='SMART_Lincoln_2009/Cdc42_and_gdp/1'>Cdc42</scene> | ||
<scene name='SMART_Lincoln_2009/Complex_intersectin_and_cdc42/1'>Cdc42/Intersectin Complex</scene> | |||
The orientation of Alanine 59 is the highlight of Cdc42 activation by Intersectin. Alanine 59 causes the displacement of the Mg++ ion which in turn releases GDP from Cdc42’s binding pocket. Cdc42 is then able to accept a new nucleotide, specifically GTP, to become activated. | The orientation of Alanine 59 is the highlight of Cdc42 activation by Intersectin. Alanine 59 causes the displacement of the Mg++ ion which in turn releases GDP from Cdc42’s binding pocket. Cdc42 is then able to accept a new nucleotide, specifically GTP, to become activated. | ||
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==<font color = 'red'>MSOE Center for BioMolecular Modeling and SMART Teams</font>== | ==<font color = 'red'>MSOE Center for BioMolecular Modeling and SMART Teams</font>== | ||
[[Image:Center for BioMolecular Modeling Logo.jpg|left|200px]] | [[Image:Center for BioMolecular Modeling Logo.jpg|left|200px]] | ||