Isochorismate pyruvate lyase: Difference between revisions
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Molecular replacement of the Apo with the structure of ECM (chorismate mutase) was done. Crystals of the bound structure are obtained by growing in PEG 3350. | Molecular replacement of the Apo with the structure of ECM (chorismate mutase) was done. Crystals of the bound structure are obtained by growing in PEG 3350. | ||
The resolution abtained for the Apo structure is 2.35 Angstroms and for the pyruvate bound structure it is 1.95 Angstroms. The residues in the loop that connects the first helix to the second has no residues lighting up in the data, depicting the disorderliness. These residues are present in case of the pyruvate bound structures in which case they are ordered. | The resolution abtained for the Apo structure is 2.35 Angstroms and for the pyruvate bound structure it is 1.95 Angstroms. The residues in the loop that connects the first helix to the second has no residues lighting up in the data, depicting the disorderliness. These residues are present in case of the pyruvate bound structures in which case they are ordered. | ||
<table width='400' align='right' cellpadding='5'><tr><td rowspan='2'> </td><td bgcolor='#eeeeee'><applet load='2h9dmorph8.pdb' size='390' frame='true' align='right' scene='User:Wayne_Decatur/Sandboxmangai/2h9d_morph/2' /></td></tr><tr><td bgcolor='#eeeeee'><center>'''Isochorismate-Pyruvate Lyase: open to closed | |||
<table width='400' align='right' cellpadding='5'><tr><td rowspan='2'> </td><td bgcolor='#eeeeee'><applet load='2h9dmorph8.pdb' size='390' frame='true' align='right' scene='User:Wayne_Decatur/Sandboxmangai/2h9d_morph/2' /></td></tr><tr><td bgcolor='#eeeeee'><center>'''Isochorismate-Pyruvate Lyase: open to closed''' (2h9dmorph8.pdb), resolution XXXÅ (<scene name='scene name='User:Wayne_Decatur/Sandboxmangai/2h9d_morph/2'>initial scene</scene>).</center></td></tr></table> | |||
Click <scene name='User:Wayne_Decatur/Sandboxmangai/2h9cmorph2h9d/2'>here to initiate the animation showing morph of aopenzyme to pyruvate bound to the open confirmation</scene>.<br> | Click <scene name='User:Wayne_Decatur/Sandboxmangai/2h9cmorph2h9d/2'>here to initiate the animation showing morph of aopenzyme to pyruvate bound to the open confirmation</scene>.<br> | ||
Click <scene name='User:Wayne_Decatur/Sandboxmangai/2h9cmorph2h9dplusside/1'>here</scene> to see sidechains in the morph too.<br> | Click <scene name='User:Wayne_Decatur/Sandboxmangai/2h9cmorph2h9dplusside/1'>here</scene> to see sidechains in the morph too.<br> | ||
To the right we can see the structure from apo to open pyruvate bound form. The representation from open to closed is given to the left. | |||
'''Isochorismate-Pyruvate Lyase: open conformation with pyruvate bound morphing to closed conformation with pyruvate bound'''<br> | |||
Click <scene name='User:Wayne_Decatur/Sandboxmangai/2h9d_morph/2'>here to initiate the animation showing morph of open conformation with pyruvate bound morphing to closed conformation with pyruvate bound</scene>.<br> | |||