Lac repressor: Difference between revisions
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==DNA Binding: Bending the Operator== | ==DNA Binding: Bending the Operator== | ||
<table width='350' align='right' cellpadding='5'><tr><td rowspan='2'> </td><td bgcolor='#d0d0d0'><applet load='1osl_19_1l1m_9_morph.pdb' size='350' frame='true' align='right' scene='Morphs/1osl_19_1l1m_9_morph/2' /></td></tr><tr><td bgcolor='#d0d0d0'>[[Morphs|Morph]] of the lac repressor bending DNA as binding changes from non-specific [[1osl]] to specific recognition of the operator sequence [[1l1m]].</td></tr></table> | <table width='350' align='right' cellpadding='5'><tr><td rowspan='2'> </td><td bgcolor='#d0d0d0'><applet load='1osl_19_1l1m_9_morph.pdb' size='350' frame='true' align='right' scene='Morphs/1osl_19_1l1m_9_morph/2' /></td></tr><tr><td bgcolor='#d0d0d0'>[[Morphs|Morph]] of the lac repressor bending DNA as binding changes from non-specific ([[1osl]]) to specific recognition of the operator sequence ([[1l1m]]).</td></tr></table> | ||
Lac repressor binds to DNA non-specifically, enabling it to slide rapidly along the DNA double helix until it encounters the lac operator sequence. Upon recognizing the specific operator sequence, the binding converts from non-specific to specific. During this conversion, the hinge region goes from a disordered loop to an alpha helix, which binds in the minor groove of the DNA. This binding opens the minor groove, bending the DNA. | Lac repressor binds to DNA non-specifically, enabling it to slide rapidly along the DNA double helix until it encounters the lac operator sequence. Upon recognizing the specific operator sequence, the binding converts from non-specific to specific. During this conversion, the hinge region goes from a disordered loop to an alpha helix, which binds in the minor groove of the DNA. This binding opens the minor groove, bending the DNA. | ||