2aut: Difference between revisions

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-[[Image:2aut.jpg|left|200px]]
+{{Seed}}
+[[Image:2aut.png|left|200px]]
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 {{STRUCTURE_2aut|  PDB=2aut  |  SCENE=  }}
-'''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''
+===Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium===
-==Overview==
+<!--
-The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
+The line below this paragraph, {{ABSTRACT_PUBMED_17570338}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17570338 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17570338}}
 ==About this Structure==
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 ==Reference==
+Structural and mutational analyses reveal the functional role of active-site Lys-154 and Asp-173 of Salmonella typhimurium AphA protein., Makde RD, Gupta GD, Mahajan SK, Kumar V, Arch Biochem Biophys. 2007 Aug 1;464(1):70-9. Epub 2007 May 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17570338 17570338]
 Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14501135 14501135]
 [[Category: Acid phosphatase]]
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 [[Category: Makde, R D.]]
 [[Category: Class-b bacterial non-specific acid phosphatase]]
+[[Category: Hydrolase]]
 [[Category: Lys154asn mutant of mature apha]]
 [[Category: Metalloenzyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:30:03 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 27 10:41:44 2008''