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| {{STRUCTURE_2olc| PDB=2olc | SCENE= }} | | {{STRUCTURE_2olc| PDB=2olc | SCENE= }} |
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| '''Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho'''
| | ===Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho=== |
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| ==Overview==
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| Trivalent holmium ions were shown to isomorphously replace magnesium ions to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex provided sufficient phasing power to allow SAD and SIRAS phasing of this previously unknown structure using the L(III) absorption edge of holmium. The structure of ADP-2Ho reveals that the two Ho ions are approximately 4 A apart and are likely to share their ligands: the phosphoryl O atoms of ADP and a water molecule. The structure determination of MTR kinase using data collected using Cu Kalpha X-radiation was also attempted. Although the heavy-atom substructure determination was successful, interpretation of the map was more challenging. The isomorphous substitution of holmium for magnesium in the MTR kinase-nucleotide complex suggests that this could be a useful phasing tool for other metal-dependent nucleotide-containing proteins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17372354}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17372354 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17372354}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Smith, G D.]] | | [[Category: Smith, G D.]] |
| [[Category: Kinase adp-2ho complex]] | | [[Category: Kinase adp-2ho complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:09:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:47:09 2008'' |