1nk0: Difference between revisions

Revision as of 13:56, 29 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1nk0.png

Template:STRUCTURE 1nk0

ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITEADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE

Template:ABSTRACT PUBMED 15035983

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:15035983

Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations., Johnson SJ, Taylor JS, Beese LS, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3895-900. Epub 2003 Mar 20. PMID:12649320

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 {{STRUCTURE_1nk0|  PDB=1nk0  |  SCENE=  }}
-'''ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE'''
+===ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE===
-==Overview==
+<!--
-Accurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four base pairs in from the primer terminus. Here we a present a structural characterization of all 12 possible mismatches captured at the growing primer terminus in the active site of a polymerase. Our observations suggest four mechanisms that lead to mismatch-induced stalling of the polymerase. Furthermore, we have observed the effects of extending a mismatch up to six base pairs from the primer terminus and find that long-range distortions in the DNA transmit the presence of the mismatch back to the enzyme active site, suggesting the structural basis for the short-term memory of replication errors.
+The line below this paragraph, {{ABSTRACT_PUBMED_15035983}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15035983 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15035983}}
 ==About this Structure==
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 ==Reference==
 Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15035983 15035983]
+Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations., Johnson SJ, Taylor JS, Beese LS, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3895-900. Epub 2003 Mar 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12649320 12649320]
 [[Category: DNA-directed DNA polymerase]]
 [[Category: Beese, L S.]]
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 [[Category: Klenow fragment]]
 [[Category: Protein-dna complex]]
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+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:56:24 2008''