2ger: Difference between revisions

Revision as of 13:42, 29 July 2008

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File:2ger.png

Template:STRUCTURE 2ger

Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate ReductaseCrystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase

Template:ABSTRACT PUBMED 16730026

About this StructureAbout this Structure

2GER is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:16730026

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OCA

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-'''Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase'''
+===Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase===
-==Overview==
+<!--
-Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.
+The line below this paragraph, {{ABSTRACT_PUBMED_16730026}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16730026 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_16730026}}
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 [[Category: Crystal structure,oxidative mechanism of human pyrroline-5-carboxylate]]
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