2f5w: Difference between revisions

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-[[Image:2f5w.gif|left|200px]]
+{{Seed}}
+[[Image:2f5w.png|left|200px]]
 <!--
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 {{STRUCTURE_2f5w|  PDB=2f5w  |  SCENE=  }}
-'''Cross-linked barnase soaked in 3 M thiourea'''
+===Cross-linked barnase soaked in 3 M thiourea===
-==Overview==
+<!--
-Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (&gt;or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).
+The line below this paragraph, {{ABSTRACT_PUBMED_16600702}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16600702 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16600702}}
 ==About this Structure==
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 [[Category: Thiourea]]
 [[Category: Urea]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:29:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:09:55 2008''