Rubrerythrin (Rr) is a non-heme iron protein isolated from anaerobic sulfate-reducing bacteria. Rr is a dimeric molecule, each monomer contains a Fe(SCys)(4) center in the C-terminal domain and a binuclear metal center in the N-terminal domain. Rr structures with different protein sources and/or preparation procedures have been studied. Two Rr crystal structures have been solved with significant differences in their binuclear metal centers. The first structure, which was obtained from expressed protein under aerobic conditions, has a diiron-oxo center. The second structure, which was obtained from native protein of Desulfovibrio vulgaris under aerobic conditions, has an Fe-Zn center with the zinc position differing from the corresponding iron position in the former structure by approximately 2 A. The crystal structures of Rr isolated from D. vulgaris (Hildenborough, NCIB 8303), the same as the second structured but prepared under anaerobic conditions, are reported in this paper. The binuclear metal center in these structures is an Fe-Zn center. When the crystal was exposed to air, the zinc atom moved gradually, approximately 2 A, accompanied by the entrance of a water molecule (or hydroxyl group) and changes in the binuclear metal center microenvironment. This finding can explain the differences between the two different structures. The results suggest that the zinc movement may be related to the enzymatic activity of Rr.
The line below this paragraph, {{ABSTRACT_PUBMED_12459910}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12459910 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12459910}}
==About this Structure==
==About this Structure==
Line 26:
Line 30:
[[Category: Liu, M Y.]]
[[Category: Liu, M Y.]]
[[Category: Alpha helix bundle]]
[[Category: Alpha helix bundle]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:04:18 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:59:42 2008''
Revision as of 10:59, 29 July 2008
This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.
