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| [[Image:2hf9.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2hf9| PDB=2hf9 | SCENE= }} | | {{STRUCTURE_2hf9| PDB=2hf9 | SCENE= }} |
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| '''Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form'''
| | ===Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form=== |
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| ==Overview==
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| HypB is a prokaryotic metal-binding guanine nucleotide-binding protein that is essential for nickel incorporation into hydrogenases. Here we solved the x-ray structure of HypB from Methanocaldococcus jannaschii. It shows that the G-domain has a different topology than the Ras-like proteins and belongs to the SIMIBI (after Signal Recognition Particle, MinD and BioD) class of NTP-binding proteins. We show that HypB undergoes nucleotide-dependent dimerization, which is apparently a common feature of SIMIBI class G-proteins. The nucleotides are located in the dimer interface and are contacted by both subunits. The active site features residues from both subunits arguing that hydrolysis also requires dimerization. Two metal-binding sites are found, one of which is dependent on the state of bound nucleotide. A totally conserved ENV/IGNLV/ICP motif in switch II relays the nucleotide binding with the metal ionbinding site. The homology with NifH, the Fe protein subunit of nitrogenase, suggests a mechanistic model for the switch-dependent incorporation of a metal ion into hydrogenases.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16807243}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16807243 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16807243}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Alpha and beta protein]] | | [[Category: Alpha and beta protein]] |
| [[Category: P-loop containing nucleoside triphosphate hydrolase]] | | [[Category: P-loop containing nucleoside triphosphate hydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:13:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:42:39 2008'' |