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| [[Image:1qfw.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1qfw| PDB=1qfw | SCENE= }} | | {{STRUCTURE_1qfw| PDB=1qfw | SCENE= }} |
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| '''TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT'''
| | ===TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT=== |
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| ==Overview==
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| Human chorionic gonadotropin (hCG), is a placental hormone which exerts its major effect by stimulating progesterone production, crucially sustaining the early weeks of pregnancy. Detection of hCG with specific monoclonal antibodies (mAbs) has become the chosen means for pregnancy diagnosis. We have used antibody Fv fragments derived from two high-affinity mAbs, one against the alpha and the other against the beta-hCG subunit to enable the crystallisation of intact or desialylated hCG. Crystals of a ternary complex composed of Fv anti-alpha/hCG/Fv anti-beta were found to diffract to 3.5 A resolution, and the structure was solved by molecular replacement. In the crystal, the two Fvs keep hCG as in a molecular cage, providing good protein-protein contacts and leaving enough space for the saccharides to be accommodated in the cell solvent. The two Fvs were found not to interact directly through their complementary-determining regions with the hCG saccharides, but only with the protein. The hCG structure in the ternary complex was very close to that of the HF partially deglycosylated hormone, thus indicating that neither the saccharides nor the Fvs had any substantial influence on hormone structure.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10373373}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10373373 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10373373}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Glycoprotein hormone]] | | [[Category: Glycoprotein hormone]] |
| [[Category: Stimulation of production of progesterone]] | | [[Category: Stimulation of production of progesterone]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:13:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:20:29 2008'' |