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| {{STRUCTURE_2vad| PDB=2vad | SCENE= }} | | {{STRUCTURE_2vad| PDB=2vad | SCENE= }} |
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| '''MONOMERIC RED FLUORESCENT PROTEIN, DSRED.M1'''
| | ===MONOMERIC RED FLUORESCENT PROTEIN, DSRED.M1=== |
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| ==Overview==
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| The red fluorescent protein DsRed has been extensively engineered for use as an in vivo research tool. In fast maturing DsRed variants, the chromophore maturation half-time is approximately 40 min, compared to approximately 12 h for wild-type DsRed. Further, DsRed has been converted from a tetramer into a monomer, a task that entailed mutating approximately 20% of the amino acids. These engineered variants of DsRed have proven extremely valuable for biomedical research, but the structural basis for the improved characteristics has not been thoroughly investigated. Here we present a 1.7 A crystal structure of the fast maturing tetrameric variant DsRed.T4. We also present a biochemical characterization and 1.6 A crystal structure of the monomeric variant DsRed.M1, also known as DsRed-Monomer. Analysis of the crystal structures suggests that rearrangements of Ser69 and Glu215 contribute to fast maturation, and that positioning of the Lys70 side chain modulates fluorescence quantum yield. Despite the 45 mutations in DsRed.M1 relative to wild-type DsRed, there is a root-mean-square deviation of only 0.3 A between the two structures. We propose that novel intramolecular interactions in DsRed.M1 partially compensate for the loss of intermolecular interactions found in the tetramer. | | The line below this paragraph, {{ABSTRACT_PUBMED_17962222}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17962222 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17962222}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Monomeric]] | | [[Category: Monomeric]] |
| [[Category: Photoprotein]] | | [[Category: Photoprotein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:29:43 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:49:11 2008'' |