2hre: Difference between revisions

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{{STRUCTURE_2hre|  PDB=2hre  |  SCENE=  }}  
{{STRUCTURE_2hre|  PDB=2hre  |  SCENE=  }}  


'''Structure of human ferrochelatase variant E343K with protoporphyrin IX bound'''
===Structure of human ferrochelatase variant E343K with protoporphyrin IX bound===




==Overview==
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Ferrochelatase, the terminal enzyme in heme biosynthesis, catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme IX. Human ferrochelatase is a homodimeric, inner mitochondrial membrane-associated enzyme that possesses an essential [2Fe-2S] cluster. In this work, we report the crystal structure of human ferrochelatase with the substrate protoporphyrin IX bound as well as a higher resolution structure of the R115L variant without bound substrate. The data presented reveal that the porphyrin substrate is bound deep within an enclosed pocket. When compared with the location of N-methylmesoporphyrin in the Bacillus subtilis ferrochelatase, the porphyrin is rotated by approximately 100 degrees and is buried an additional 4.5 A deeper within the active site. The propionate groups of the substrate do not protrude into solvent and are bound in a manner similar to what has been observed in uroporphyrinogen decarboxylase. Furthermore, in the substrate-bound form, the jaws of the active site mouth are closed so that the porphyrin substrate is completely engulfed in the pocket. These data provide insights that will aid in the determination of the mechanism for ferrochelatase.
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==Disease==
==Disease==
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[[Category: Heme synthesis]]
[[Category: Heme synthesis]]
[[Category: Protoporphyrin ix]]
[[Category: Protoporphyrin ix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:37:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:40:18 2008''

Revision as of 09:40, 29 July 2008

File:2hre.png

Template:STRUCTURE 2hre

Structure of human ferrochelatase variant E343K with protoporphyrin IX boundStructure of human ferrochelatase variant E343K with protoporphyrin IX bound

Template:ABSTRACT PUBMED 17261801

DiseaseDisease

Known disease associated with this structure: Protoporphyria, erythropoietic OMIM:[177000], Protoporphyria, erythropoietic, recessive, with liver failure OMIM:[177000]

About this StructureAbout this Structure

2HRE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Substrate interactions with human ferrochelatase., Medlock A, Swartz L, Dailey TA, Dailey HA, Lanzilotta WN, Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1789-93. Epub 2007 Jan 29. PMID:17261801

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