'''Crystal structure of the complex of sheep signalling glycoprotein with chitin trimer at 3.0A resolution'''
===Crystal structure of the complex of sheep signalling glycoprotein with chitin trimer at 3.0A resolution===
==Overview==
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Crystal structures of four complexes of sheep secretory glycoprotein (SPS-40) with N-acetylglucosamine oligosaccharides (GlcNAc(n), (n=3-6)) have been determined at moderate resolutions. The binding studies of SPS-40 have been carried out using fluorescence spectroscopy and Surface Plasmon Resonance (SPR). Structure determinations of four complexes have shown a novel binding pattern of GlcNAc(n) molecules to SPS-40. The results indicate that the most preferred recognition region in the carbohydrate binding groove in SPS-40 is at subsites -4 to -2 among which subsite -2 provides the maximum interactions with carbohydrate residues. These structures have also shown that the interactions of GlcNAc3 and GlcNAc4 do not perturb the protein structure and those of GlcNAc5 induce partial conformational changes while in the case of GlcNAc6 the partially closed binding groove opened up completely. As in other SPX-40 structures, SPS-40 structure contains three overlapping flexible surface segments, His188-His197, Phe202-Arg212 and Phe244-Pro260 with several charged residues protruding outwardly. It creates a cluster of positive charges with a flexible base thus indicating a good scope of promoting the intermolecular interactions. This protein is glycosylated at Asn39 and may recognize other receptors having sugar binding sites. It appears that SPS-40 may involve both carbohydrate and protein bindings. The systematic carbohydrate-binding studies and the detailed structural results of four protein-carbohydrate complexes provide an excellent insight into the mechanism of carbohydrate binding. These are the first studies of this kind on secretory glycoproteins and their interactions with carbohydrates.
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{{ABSTRACT_PUBMED_17188513}}
==About this Structure==
==About this Structure==
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[[Category: Sps-40]]
[[Category: Sps-40]]
[[Category: Trisaccharide]]
[[Category: Trisaccharide]]
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Revision as of 09:39, 29 July 2008
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Crystal structure of the complex of sheep signalling glycoprotein with chitin trimer at 3.0A resolutionCrystal structure of the complex of sheep signalling glycoprotein with chitin trimer at 3.0A resolution
