1r4q: Difference between revisions

Revision as of 09:02, 29 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1r4q.png

Template:STRUCTURE 1r4q

Shiga toxinShiga toxin

Template:ABSTRACT PUBMED 15075327

About this StructureAbout this Structure

1R4Q is a Protein complex structure of sequences from Shigella dysenteriae. Full crystallographic information is available from OCA.

ReferenceReference

Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7., Fraser ME, Fujinaga M, Cherney MM, Melton-Celsa AR, Twiddy EM, O'Brien AD, James MN, J Biol Chem. 2004 Jun 25;279(26):27511-7. Epub 2004 Apr 9. PMID:15075327

Page seeded by OCA on Tue Jul 29 09:02:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1r4q.gif|left|200px]]
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-'''Shiga toxin'''
+===Shiga toxin===
-==Overview==
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-Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are all AB(5) toxins, the crystal structure of Stx2 from E. coli O157:H7 was determined at 1.8-A resolution and compared with the known structure of Stx. Our major finding was that, in contrast to Stx, the active site of the A-subunit of Stx2 is accessible in the holotoxin, and a molecule of formic acid and a water molecule mimic the binding of the adenine base of the substrate. Further, the A-subunit adopts a different orientation with respect to the B-subunits in Stx2 than in Stx, due to interactions between the carboxyl termini of the B-subunits and neighboring regions of the A-subunit. Of the three types of receptor-binding sites in the B-pentamer, one has a different conformation in Stx2 than in Stx, and the carboxyl terminus of the A-subunit binds at another. Any of these structural differences might result in different mechanisms of action of the two toxins and the development of hemolytic uremic syndrome upon exposure to Stx2.
+The line below this paragraph, {{ABSTRACT_PUBMED_15075327}}, adds the Publication Abstract to the page
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+-->
+{{ABSTRACT_PUBMED_15075327}}
 ==About this Structure==
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 [[Category: Twiddy, E M.]]
 [[Category: Ab5 toxin]]
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