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| [[Image:2nxn.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2nxn| PDB=2nxn | SCENE= }} | | {{STRUCTURE_2nxn| PDB=2nxn | SCENE= }} |
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| '''T. thermophilus ribosomal protein L11 methyltransferase (PrmA) in complex with ribosomal protein L11'''
| | ===T. thermophilus ribosomal protein L11 methyltransferase (PrmA) in complex with ribosomal protein L11=== |
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| ==Overview==
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| Bacterial ribosomal protein L11 is post-translationally trimethylated at multiple residues by a single methyltransferase, PrmA. Here, we describe four structures of PrmA from the extreme thermophile Thermus thermophilus. Two apo-PrmA structures at 1.59 and 2.3 A resolution and a third with bound cofactor S-adenosyl-L-methionine at 1.75 A each exhibit distinct relative positions of the substrate recognition and catalytic domains, revealing how PrmA can position the L11 substrate for multiple, consecutive side-chain methylation reactions. The fourth structure, the PrmA-L11 enzyme-substrate complex at 2.4 A resolution, illustrates the highly specific interaction of the N-terminal domain with its substrate and places Lys39 in the PrmA active site. The presence of a unique flexible loop in the cofactor-binding site suggests how exchange of AdoMet with the reaction product S-adenosyl-L-homocysteine can occur without necessitating the dissociation of PrmA from L11. Finally, the mode of interaction of PrmA with L11 explains its observed preference for L11 as substrate before its assembly into the 50S ribosomal subunit.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17215866}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17215866 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17215866}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Post-translational modification]] | | [[Category: Post-translational modification]] |
| [[Category: S-adenosyl-l-methionine dependent methyltransferase]] | | [[Category: S-adenosyl-l-methionine dependent methyltransferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:02:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:43:27 2008'' |