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| {{STRUCTURE_1n8v| PDB=1n8v | SCENE= }} | | {{STRUCTURE_1n8v| PDB=1n8v | SCENE= }} |
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| '''Chemosensory Protein in complex with bromo-dodecanol'''
| | ===Chemosensory Protein in complex with bromo-dodecanol=== |
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| ==Overview==
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| Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12697900}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12697900 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12697900}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Tegoni, M.]] | | [[Category: Tegoni, M.]] |
| [[Category: Lipid binding protein]] | | [[Category: Lipid binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:14:17 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:04:06 2008'' |