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| [[Image:2nq9.gif|left|200px]] | | {{Seed}} |
| | [[Image:2nq9.png|left|200px]] |
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| {{STRUCTURE_2nq9| PDB=2nq9 | SCENE= }} | | {{STRUCTURE_2nq9| PDB=2nq9 | SCENE= }} |
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| '''High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA'''
| | ===High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA=== |
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| ==Overview==
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| Escherichia coli endonuclease IV is an archetype for an abasic or apurinic-apyrimidinic endonuclease superfamily crucial for DNA base excision repair. Here biochemical, mutational and crystallographic characterizations reveal a three-metal ion mechanism for damage binding and incision. The 1.10-A resolution DNA-free and the 2.45-A resolution DNA-substrate complex structures capture substrate stabilization by Arg37 and reveal a distorted Zn(3)-ligand arrangement that reverts, after catalysis, to an ideal geometry suitable to hold rather than release cleaved DNA product. The 1.45-A resolution DNA-product complex structure shows how Tyr72 caps the active site, tunes its dielectric environment and promotes catalysis by Glu261-activated hydroxide, bound to two Zn(2+) ions throughout catalysis. These structural, mutagenesis and biochemical results suggest general requirements for abasic site removal in contrast to features specific to the distinct endonuclease IV alpha-beta triose phosphate isomerase (TIM) barrel and APE1 four-layer alpha-beta folds of the apurinic-apyrimidinic endonuclease families.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18408731}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18408731 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18408731}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Tim-barrel]] | | [[Category: Tim-barrel]] |
| [[Category: Trinuclear zn active site]] | | [[Category: Trinuclear zn active site]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:25:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:26:30 2008'' |