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| [[Image:2efw.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2efw| PDB=2efw | SCENE= }} | | {{STRUCTURE_2efw| PDB=2efw | SCENE= }} |
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| '''Crystal structure of the RTP:nRB complex from Bacillus subtilis'''
| | ===Crystal structure of the RTP:nRB complex from Bacillus subtilis=== |
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| ==Overview==
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| In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. We report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. The 21 bp RTP:DNA complex displays marked structural asymmetry in both the homodimeric protein and the DNA. This is in contrast to the previously reported complex formed with a symmetrical TerI B-site homologue. The induced asymmetry is consistent with the complex's solution properties as determined using NMR spectroscopy. Concomitant with this asymmetry is variation in the protein:DNA binding pattern for each of the subunits of the RTP homodimer. It is proposed that the asymmetric "wing" positions, as well as other asymmetrical features of the RTP:DNA complex, are critical for the cooperative binding that underlies the mechanism of polar fork arrest at the complete terminator site.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17521668}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17521668 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17521668}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Replication fork arrest]] | | [[Category: Replication fork arrest]] |
| [[Category: Replication/dna complex]] | | [[Category: Replication/dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:29:20 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:52:31 2008'' |