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| [[Image:2il8.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2il8| PDB=2il8 | SCENE= }} | | {{STRUCTURE_2il8| PDB=2il8 | SCENE= }} |
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| '''THREE-DIMENSIONAL STRUCTURE OF INTERLEUKIN 8 IN SOLUTION'''
| | ===THREE-DIMENSIONAL STRUCTURE OF INTERLEUKIN 8 IN SOLUTION=== |
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| ==Overview==
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| The solution structure of the interleukin 8 (IL-8) dimer has been solved by nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-dynamical simulated annealing calculations. The structure determination is based on a total of 1880 experimental distance restraints (of which 82 are intersubunit) and 362 torsion angle restraints (comprising phi, psi, and chi 1 torsion angles). A total of 30 simulated annealing structures were calculated, and the atomic rms distribution about the mean coordinate positions (excluding residues 1-5 of each subunit) is 0.41 +/- 0.08 A for the backbone atoms and 0.90 +/- 0.08 A for all atoms. The three-dimensional solution structure of the IL-8 dimer reveals a structural motif in which two symmetry-related antiparallel alpha-helices, approximately 24 A long and separated by about 14 A, lie on top of a six-stranded antiparallel beta-sheet platform derived from two three-stranded Greek keys, one from each monomer unit. The general architecture is similar to that of the alpha 1/alpha 2 domains of the human class I histocompatibility antigen HLA-A2. It is suggested that the two alpha-helices form the binding site for the cellular receptor and that the specificity of IL-8, as well as that of a number of related proteins involved in cell-specific chemotaxis, mediation of cell growth, and the inflammatory response, is achieved by the distinct distribution of charged and polar residues at the surface of the helices.
| | The line below this paragraph, {{ABSTRACT_PUBMED_2184886}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 2184886 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_2184886}} |
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| ==About this Structure== | | ==About this Structure== |
| 2IL8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IL8 OCA]. | | 2IL8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IL8 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Clore, G M.]] | | [[Category: Clore, G M.]] |
| [[Category: Cytokine]] | | [[Category: Cytokine]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:37:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:18:17 2008'' |