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| [[Image:1tv3.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1tv3| PDB=1tv3 | SCENE= }} | | {{STRUCTURE_1tv3| PDB=1tv3 | SCENE= }} |
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| '''Crystal structure of the N-methyl-hydroxylamine MtmB complex'''
| | ===Crystal structure of the N-methyl-hydroxylamine MtmB complex=== |
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| ==Overview==
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| L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15380192}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15380192 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15380192}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Zhao, G.]] | | [[Category: Zhao, G.]] |
| [[Category: Tim barrel]] | | [[Category: Tim barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:24:14 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:48:04 2008'' |