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| [[Image:2gg6.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2gg6| PDB=2gg6 | SCENE= }} | | {{STRUCTURE_2gg6| PDB=2gg6 | SCENE= }} |
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| '''CP4 EPSP synthase liganded with S3P'''
| | ===CP4 EPSP synthase liganded with S3P=== |
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| ==Overview==
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| The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation. | | The line below this paragraph, {{ABSTRACT_PUBMED_16916934}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16916934 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16916934}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Inside-out alpha/beta barrel]] | | [[Category: Inside-out alpha/beta barrel]] |
| [[Category: Two domain structure]] | | [[Category: Two domain structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:04:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:08:04 2008'' |