1ysd: Difference between revisions

Revision as of 03:15, 29 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1ysd.png

Template:STRUCTURE 1ysd

Yeast Cytosine Deaminase Double MutantYeast Cytosine Deaminase Double Mutant

Template:ABSTRACT PUBMED 15879217

About this StructureAbout this Structure

1YSD is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Computational thermostabilization of an enzyme., Korkegian A, Black ME, Baker D, Stoddard BL, Science. 2005 May 6;308(5723):857-60. PMID:15879217

Page seeded by OCA on Tue Jul 29 03:15:44 2008

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OCA

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-'''Yeast Cytosine Deaminase Double Mutant'''
+===Yeast Cytosine Deaminase Double Mutant===
-==Overview==
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-Thermostabilizing an enzyme while maintaining its activity for industrial or biomedical applications can be difficult with traditional selection methods. We describe a rapid computational approach that identified three mutations within a model enzyme that produced a 10 degrees C increase in apparent melting temperature T(m) and a 30-fold increase in half-life at 50 degrees C, with no reduction in catalytic efficiency. The effects of the mutations were synergistic, giving an increase in excess of the sum of their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required its activity, thereby coupling molecular and metabolic engineering.
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 ==About this Structure==
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 [[Category: Cytosine deaminase]]
 [[Category: Hydrolase]]
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