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| [[Image:1yef.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1yef| PDB=1yef | SCENE= }} | | {{STRUCTURE_1yef| PDB=1yef | SCENE= }} |
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| '''STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE'''
| | ===STRUCTURE OF IGG2A FAB FRAGMENT (D2.3) COMPLEXED WITH SUBSTRATE ANALOGUE=== |
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| ==Overview==
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| The x-ray structures of the unliganded esterase-like catalytic antibody D2.3 and its complexes with a substrate analogue and with one of the reaction products are analyzed. Together with the structure of the phosphonate transition state analogue hapten complex, these crystal structures provide a complete description of the reaction pathway. At alkaline pH, D2.3 acts by preferential stabilization of the negatively charged oxyanion intermediate of the reaction that results from hydroxide attack on the substrate. A tyrosine residue plays a crucial role in catalysis: it activates the ester substrate and, together with an asparagine, it stabilizes the oxyanion intermediate. A canal allows facile diffusion of water molecules to the reaction center that is deeply buried in the structure. Residues bordering this canal provide targets for mutagenesis to introduce a general base in the vicinity of the reaction center. | | The line below this paragraph, {{ABSTRACT_PUBMED_9223277}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9223277 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9223277}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Knossow, M.]] | | [[Category: Knossow, M.]] |
| [[Category: Catalytic antibody]] | | [[Category: Catalytic antibody]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:13:17 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:08:27 2008'' |