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| {{STRUCTURE_2g6x| PDB=2g6x | SCENE= }} | | {{STRUCTURE_2g6x| PDB=2g6x | SCENE= }} |
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| '''Crystal structure of a novel green fluorescent protein from marine copepod Pontellina plumata'''
| | ===Crystal structure of a novel green fluorescent protein from marine copepod Pontellina plumata=== |
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| ==Overview==
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| Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16936637}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16936637 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16936637}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Natural chromophore]] | | [[Category: Natural chromophore]] |
| [[Category: Rapid maturation]] | | [[Category: Rapid maturation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:45:33 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:53:35 2008'' |