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| {{STRUCTURE_1woc| PDB=1woc | SCENE= }} | | {{STRUCTURE_1woc| PDB=1woc | SCENE= }} |
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| '''Crystal structure of PriB'''
| | ===Crystal structure of PriB=== |
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| ==Overview==
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| PriB is not only an essential protein necessary for the replication restart on the collapsed and disintegrated replication fork, but also an important protein for assembling of primosome onto PhiX174 genomic DNA during replication initiation. Here we report a 2.0-A-resolution X-ray structure of a biologically functional form of PriB from Escherichia coli. The crystal structure revealed that despite a low level of primary sequence identity, the PriB monomer, as well as the dimeric form, are structurally identical to the N-terminal DNA-binding domain of the single-stranded DNA-binding protein (SSB) from Escherichia coli, which possesses an oligonucleotides-binding-fold. The oligonucleotide-PriB complex model based on the oligonucleotides-SSB complex structure suggested that PriB had a DNA-binding pocket conserved in SSB from Escherichia coli and might bind to single-stranded DNA in the manner of SSB. Furthermore, surface plasmon resonance analysis and fluorescence measurements demonstrated that PriB binds single-stranded DNA with high affinity, by involving tryptophan residue. The significance of these results with respect to the functional role of PriB in the assembly of primosome is discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15607735}}, adds the Publication Abstract to the page |
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| | {{ABSTRACT_PUBMED_15607735}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ueda, T.]] | | [[Category: Ueda, T.]] |
| [[Category: Oligonucleotide binding fold]] | | [[Category: Oligonucleotide binding fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:56:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:45:34 2008'' |