1xsu: Difference between revisions

Revision as of 02:34, 29 July 2008

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File:1xsu.png

Template:STRUCTURE 1xsu

Solution structure of E.coli RNase P RNA P4 stem, U69C/C70U mutation, complexed with cobalt (III) hexammine.Solution structure of E.coli RNase P RNA P4 stem, U69C/C70U mutation, complexed with cobalt (III) hexammine.

Template:ABSTRACT PUBMED 15576680

About this StructureAbout this Structure

Full experimental information is available from OCA.

ReferenceReference

Change of RNase P RNA function by single base mutation correlates with perturbation of metal ion binding in P4 as determined by NMR spectroscopy., Schmitz M, Nucleic Acids Res. 2004 Dec 2;32(21):6358-66. Print 2004. PMID:15576680

Page seeded by OCA on Tue Jul 29 02:34:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Solution structure of E.coli RNase P RNA P4 stem, U69C/C70U mutation, complexed with cobalt (III) hexammine.'''
+===Solution structure of E.coli RNase P RNA P4 stem, U69C/C70U mutation, complexed with cobalt (III) hexammine.===
-==Overview==
+<!--
-The solution structures of two 27 nt RNA hairpins and their complexes with cobalt(III)-hexammine [Co(NH(3))(6)(3+)] were determined by NMR spectroscopy. The RNA hairpins are variants of the P4 region from Escherichia coli RNase P RNA: a U-to-A mutant changing the identity of the bulged nucleotide, and a U-to-C, C-to-U double mutant changing only the bulge position. Structures calculated from NMR constraints show that the RNA hairpins adopt different conformations. In the U-to-C, C-to-U double mutant, the conserved bulged uridine in the P4 wild-type stem is found to be shifted in the 3'-direction by one nucleotide when compared with the wild-type structure. Co(NH(3))(6)(3+) is used as a spectroscopic probe for Mg(H(2)O)(6)(2+) binding sites because both complexes have octahedral symmetry and have similar radii. Intermolecular NOE crosspeaks between Co(NH(3))(6)(3+) and RNA protons were used to locate the site of Co(NH(3))(6)(3+) binding to both RNA hairpins. The metal ion binds in the major groove near a bulge loop in both mutants, but is shifted 3' by about one base pair in the double mutant. The change of the metal ion binding site is compared with results obtained on corresponding mutant RNase P RNA molecules as reported by Harris and co-workers (RNA, 1, 210-218).
+The line below this paragraph, {{ABSTRACT_PUBMED_15576680}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15576680 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15576680}}
 ==About this Structure==
-Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSU OCA].
+Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSU OCA].
 ==Reference==
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 [[Category: Transfer rna processing]]
 [[Category: U69c/c70u mutant]]
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+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:34:30 2008''