1tuh: Difference between revisions

Revision as of 02:15, 29 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1tuh.png

Template:STRUCTURE 1tuh

Structure of Bal32a from a Soil-Derived Mobile Gene CassetteStructure of Bal32a from a Soil-Derived Mobile Gene Cassette

Template:ABSTRACT PUBMED 15713477

About this StructureAbout this Structure

1TUH is a Single protein structure of sequence from Uncultured bacterium. Full crystallographic information is available from OCA.

ReferenceReference

Integron-associated mobile gene cassettes code for folded proteins: the structure of Bal32a, a new member of the adaptable alpha+beta barrel family., Robinson A, Wu PS, Harrop SJ, Schaeffer PM, Dosztanyi Z, Gillings MR, Holmes AJ, Nevalainen KM, Stokes HW, Otting G, Dixon NE, Curmi PM, Mabbutt BC, J Mol Biol. 2005 Mar 11;346(5):1229-41. Epub 2004 Dec 31. PMID:15713477

Page seeded by OCA on Tue Jul 29 02:15:03 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1tuh.gif|left|200px]]
+{{Seed}}
+[[Image:1tuh.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1tuh|  PDB=1tuh  |  SCENE=  }}
-'''Structure of Bal32a from a Soil-Derived Mobile Gene Cassette'''
+===Structure of Bal32a from a Soil-Derived Mobile Gene Cassette===
-==Overview==
+<!--
-The wide-ranging physiology and large genetic variability observed for prokaryotes is largely attributed, not to the prokaryotic genome itself, but rather to mechanisms of lateral gene transfer. Cassette PCR has been used to sample the integron/gene cassette metagenome from different natural environments without laboratory cultivation of the host organism, and without prior knowledge of any target protein sequence. Since over 90% of cassette genes are unrelated to any sequence in the current databases, it is not clear whether these genes code for folded functional proteins. We have selected a sample of eight cassette-encoded genes with no known homologs; five have been isolated as soluble protein products and shown by biophysical techniques to be folded. In solution, at least three of these proteins organise as stable oligomeric assemblies. The tertiary structure of one of these, Bal32a derived from a contaminated soil site, has been solved by X-ray crystallography to 1.8 A resolution. From the three-dimensional structure, Bal32a is found to be a member of the highly adaptable alpha+beta barrel family of transport proteins and enzymes. In Bal32a, the barrel cavity is unusually deep and inaccessible to solvent. Polar side-chains in its interior are reminiscent of catalytic sites of limonene-1,2-epoxide hydrolase and nogalonic acid methyl ester cyclase. These studies demonstrate the viability of direct sampling of mobile DNA as a route for the discovery of novel proteins.
+The line below this paragraph, {{ABSTRACT_PUBMED_15713477}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15713477 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15713477}}
 ==About this Structure==
@@ Line 35: / Line 39: @@
 [[Category: Wu, P S.C.]]
 [[Category: Uncultured]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:22:53 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:15:03 2008''