2oqu: Difference between revisions

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{{STRUCTURE_2oqu|  PDB=2oqu  |  SCENE=  }}  
{{STRUCTURE_2oqu|  PDB=2oqu  |  SCENE=  }}  


'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
===High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths===




==Overview==
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Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
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==About this Structure==
==About this Structure==
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[[Category: Sad phasing]]
[[Category: Sad phasing]]
[[Category: Xenon]]
[[Category: Xenon]]
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Revision as of 01:37, 29 July 2008

File:2oqu.png

Template:STRUCTURE 2oqu

High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long WavelengthsHigh Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths

Template:ABSTRACT PUBMED 17452791

About this StructureAbout this Structure

2OQU is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791

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