1sl1: Difference between revisions

Revision as of 00:50, 29 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1sl1.png

Template:STRUCTURE 1sl1

Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the templateBinary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template

Template:ABSTRACT PUBMED 15235589

About this StructureAbout this Structure

1SL1 is a Protein complex structure of sequences from Enterobacteria phage t7 and Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7., Li Y, Dutta S, Doublie S, Bdour HM, Taylor JS, Ellenberger T, Nat Struct Mol Biol. 2004 Aug;11(8):784-90. Epub 2004 Jul 4. PMID:15235589

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-'''Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template'''
+===Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template===
-==Overview==
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-Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.
+The line below this paragraph, {{ABSTRACT_PUBMED_15235589}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_15235589}}
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 [[Category: Open]]
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