1n87: Difference between revisions

Revision as of 22:39, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1n87.png

Template:STRUCTURE 1n87

Solution structure of the U-box of Prp19Solution structure of the U-box of Prp19

Template:ABSTRACT PUBMED 12627222

About this StructureAbout this Structure

1N87 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

ReferenceReference

Structural insights into the U-box, a domain associated with multi-ubiquitination., Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL, Nat Struct Biol. 2003 Apr;10(4):250-5. PMID:12627222

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-[[Image:1n87.gif|left|200px]]
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 {{STRUCTURE_1n87|  PDB=1n87  |  SCENE=  }}
-'''Solution structure of the U-box of Prp19'''
+===Solution structure of the U-box of Prp19===
-==Overview==
+<!--
-The structure of the U-box in the essential Saccharomyces cerevisiae pre-mRNA splicing factor Prp19p has been determined by NMR. The conserved zinc-binding sites supporting the cross-brace arrangement in RING-finger domains are replaced by hydrogen-bonding networks in the U-box. These hydrogen-bonding networks are necessary for the structural stabilization and activity of the U-box. A conservative Val--&gt;Ile point mutation in the Prp19p U-box domain leads to pre-mRNA splicing defects in vivo. NMR analysis of this mutant shows that the substitution disrupts structural integrity of the U-box domain. Furthermore, comparison of the Prp19p U-box domain with known RING-E2 complex structures demonstrates that both U-box and RING-fingers contain a conserved interaction surface. Mutagenesis of residues at this interface, while not perturbing the structure of the U-box, abrogates Prp19p function in vivo. These comparative structural and functional analyses imply that the U-box and its associated ubiquitin ligase activity are critical for Prp19p function in vivo.
+The line below this paragraph, {{ABSTRACT_PUBMED_12627222}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12627222 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_12627222}}
 ==About this Structure==
-N87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N87 OCA].
+N87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N87 OCA].
 ==Reference==
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 [[Category: U-box]]
 [[Category: Ubiquitin ligase]]
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