|
|
| Line 1: |
Line 1: |
| [[Image:1r5c.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1r5c.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1r5c| PDB=1r5c | SCENE= }} | | {{STRUCTURE_1r5c| PDB=1r5c | SCENE= }} |
|
| |
|
| '''X-ray structure of the complex of Bovine seminal ribonuclease swapping dimer with d(CpA)'''
| | ===X-ray structure of the complex of Bovine seminal ribonuclease swapping dimer with d(CpA)=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Bovine seminal ribonuclease (BS-RNase) is a unique member of the pancreatic-like ribonuclease superfamily. This enzyme exists as two conformational isomers with distinctive biological properties. The structure of the major isomer is characterized by the swapping of the N-terminal segment (MxM BS-RNase). In this article, the crystal structures of the ligand-free MxM BS-RNase and its complex with 2'-deoxycitidylyl(3',5')-2'-deoxyadenosine derived from isomorphous crystals have been refined. Interestingly, the comparison between this novel ligand-free form and the previously published sulfate-bound structure reveals significant differences. In particular, the ligand-free MxM BS-RNase is closer to the structure of MxM BS-RNase productive complexes than to the sulfate-bound form. These results reveal that MxM BS-RNase presents a remarkable flexibility, despite the structural constraints of the interchain disulfide bridges and the swapping of the N-terminal helices. These findings have important implications to the ligand binding mechanism of MxM BS-RNase. Indeed, a population shift rather than a substrate-induced conformational transition may occur in the MxM BS-RNase ligand binding process.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15048772}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15048772 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15048772}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 35: |
Line 39: |
| [[Category: Ribonuclease]] | | [[Category: Ribonuclease]] |
| [[Category: X-ray diffraction]] | | [[Category: X-ray diffraction]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:05:58 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:07:09 2008'' |