2hnf: Difference between revisions

No edit summary
No edit summary
Line 1: Line 1:
[[Image:2hnf.gif|left|200px]]
{{Seed}}
[[Image:2hnf.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hnf|  PDB=2hnf  |  SCENE=  }}  
{{STRUCTURE_2hnf|  PDB=2hnf  |  SCENE=  }}  


'''Structure of a Hyper-cleavable Monomeric Fragment of Phage lambda Repressor Containing the Cleavage Site Region'''
===Structure of a Hyper-cleavable Monomeric Fragment of Phage lambda Repressor Containing the Cleavage Site Region===




==Overview==
<!--
The key event in the switch from lysogenic to lytic growth of phage lambda is the self-cleavage of lambda repressor, which is induced by the formation of a RecA-ssDNA-ATP filament at a site of DNA damage. Lambda repressor cleaves itself at the peptide bond between Ala111 and Gly112, but only when bound as a monomer to the RecA-ssDNA-ATP filament. Here we have designed a hyper-cleavable fragment of lambda repressor containing the hinge and C-terminal domain (residues 101-229), in which the monomer-monomer interface is disrupted by two point mutations and a deletion of seven residues at the C terminus. This fragment crystallizes as a monomer and its structure has been determined to 1.8 A resolution. The hinge region, which bears the cleavage site, is folded over the active site of the C-terminal oligomerization domain (CTD) but with the cleavage site flipped out and exposed to solvent. Thus, the structure represents a non-cleavable conformation of the repressor, but one that is poised for cleavage after modest rearrangements that are presumably stabilized by binding to RecA. The structure provides a unique snapshot of lambda repressor in a conformation that sheds light on how its self-cleavage is tempered in the absence of RecA, as well as a framework for interpreting previous genetic and biochemical data concerning the RecA-mediated cleavage reaction.
The line below this paragraph, {{ABSTRACT_PUBMED_16934834}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16934834 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16934834}}


==About this Structure==
==About this Structure==
Line 25: Line 29:
[[Category: Ndjonka, D.]]
[[Category: Ndjonka, D.]]
[[Category: Virus/viral protein]]
[[Category: Virus/viral protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:29:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:59:36 2008''

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA