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| [[Image:1twc.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1twc| PDB=1twc | SCENE= }} | | {{STRUCTURE_1twc| PDB=1twc | SCENE= }} |
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| '''RNA polymerase II complexed with GTP'''
| | ===RNA polymerase II complexed with GTP=== |
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| ==Overview==
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| Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15537538}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15537538 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15537538}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Transcription]] | | [[Category: Transcription]] |
| [[Category: Zinc motif]] | | [[Category: Zinc motif]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:27:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:31:24 2008'' |