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| [[Image:1wb2.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1wb2| PDB=1wb2 | SCENE= }} | | {{STRUCTURE_1wb2| PDB=1wb2 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF TRANSLATION ELONGATION FACTOR SELB FROM METHANOCOCCUS MARIPALUDIS, APO FORM'''
| | ===CRYSTAL STRUCTURE OF TRANSLATION ELONGATION FACTOR SELB FROM METHANOCOCCUS MARIPALUDIS, APO FORM=== |
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| ==Overview==
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| In all three kingdoms of life, SelB is a specialized translation elongation factor responsible for the cotranslational incorporation of selenocysteine into proteins by recoding of a UGA stop codon in the presence of a downstream mRNA hairpin loop. Here, we present the X-ray structures of SelB from the archaeon Methanococcus maripaludis in the apo-, GDP- and GppNHp-bound form and use mutational analysis to investigate the role of individual amino acids in its aminoacyl-binding pocket. All three SelB structures reveal an EF-Tu:GTP-like domain arrangement. Upon binding of the GTP analogue GppNHp, a conformational change of the Switch 2 region in the GTPase domain leads to the exposure of SelB residues involved in clamping the 5' phosphate of the tRNA. A conserved extended loop in domain III of SelB may be responsible for specific interactions with tRNA(Sec) and act as a ruler for measuring the extra long acceptor arm. Domain IV of SelB adopts a beta barrel fold and is flexibly tethered to domain III. The overall domain arrangement of SelB resembles a 'chalice' observed so far only for initiation factor IF2/eIF5B. In our model of SelB bound to the ribosome, domain IV points towards the 3' mRNA entrance cleft ready to interact with the downstream secondary structure element.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15616587}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15616587 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15616587}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Thanbichler, M.]] | | [[Category: Thanbichler, M.]] |
| [[Category: Selenocysteine,protein synthesis,selenium,ribosome]] | | [[Category: Selenocysteine,protein synthesis,selenium,ribosome]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:24:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:19:56 2008'' |