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| [[Image:2gio.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2gio| PDB=2gio | SCENE= }} | | {{STRUCTURE_2gio| PDB=2gio | SCENE= }} |
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| '''Solution Structure of a portion of the 5'UTR of HspA mRNA of Bradyrhizobium japonicum'''
| | ===Solution Structure of a portion of the 5'UTR of HspA mRNA of Bradyrhizobium japonicum=== |
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| ==Overview==
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| Regulatory RNA elements, like riboswitches, respond to intracellular signals by three-dimensional (3D) conformational changes. RNA thermometers employ a similar strategy to sense temperature changes in the cell and regulate the translational machinery. We present here the first 3D NMR structure of the functional domain of a highly conserved bacterial RNA thermometer containing the ribosome binding site that remains occluded at normal temperatures (30 degrees C). We identified a region adjacent to the Shine-Dalgarno sequence that has a network of weak hydrogen bonds within the RNA helix. With the onset of heat shock at 42 degrees C, destabilisation of the RNA structure initiates at this region and favours the release of the ribosome binding site and of the start codon. Deletion of a highly conserved G residue leads to the formation of a stable regular RNA helix that loses thermosensing ability. Our results indicate that RNA thermometers are able to sense temperature changes without the aid of accessory factors.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16710302}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16710302 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16710302}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIO OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIO OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Narberhaus, F.]] | | [[Category: Narberhaus, F.]] |
| [[Category: G-g non cannonical pair]] | | [[Category: G-g non cannonical pair]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:09:01 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:00:28 2008'' |