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| {{STRUCTURE_2p6r| PDB=2p6r | SCENE= }} | | {{STRUCTURE_2p6r| PDB=2p6r | SCENE= }} |
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| '''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA'''
| | ===Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA=== |
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| ==Overview==
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| To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17558417}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17558417 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17558417}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| [[Category: Sf2 helicase]] | | [[Category: Sf2 helicase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:29:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:26:46 2008'' |